APOL3 Antibody

Western Blot: APOL3 Antibody [NBP1-06979] - NBP1-06979 (1ug/ml) staining of DAUDI lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

APOL3 Antibody Summary

• Immunogen: Peptide with sequence ELTQIYQRLNPCHTH corresponding to C-Terminus according to NP_663615.1, NP_055164.1, NP_663616.1.
• Specificity: This antibody is expected to recognize isoform 1 (NP_663615.1), isoform 2 (NP_055164.1, NP_085147.1, NP_663614.1) and isoform 3 (NP_663616.1, NP_663617.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: APOL3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:1000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 40 kDa band observed in lysates of DAUDI and MOLT4 (calculated MW of 44.3 kDa band according to NP_663615.1).
• Theoretical MW: 44 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  APOL3 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for APOL3 Antibody

• APOLIII
• apoL-III
• apolipoprotein L, 3
• apolipoprotein L3
• Apolipoprotein L-III
• CG12_1
• CG121
• CG12-1
• TNF-inducible protein CG12-1

Background

This gene is a member of the apolipoprotein L gene family. The encoded protein is found in the cytoplasm, where it may affect the movement of lipids or allow the binding of lipids to organelles. In addition, expression of this gene is upregulated by tumor necrosis factor-alpha in endothelial cells lining the normal and atherosclerotic iliac artery and aorta. Six transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for APOL3 Antibody (NBP1-06979)(1)

Publication using NBP1-06979

• Monajemi H, Fontijn RD, Pannekoek H, Horrevoets AJ. The apolipoprotein L gene cluster has emerged recently in evolution and is expressed in human vascular tissue. Genomics 2002-04-01 [PMID: 11944986]

Bioinformatics

• Gene Symbol: APOL3
• Entrez:
  • 80833(Human)
• Uniprot:
  • NP_663615.1(Human)