KIDINS220 Antibody (3-B6)

Western Blot: KIDINS220 Antibody (3-B6) [NBP2-66916] - Western blot analysis of kinase D-interacting substrate 220 on K562 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/100) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/Immunofluorescence: KIDINS220 Antibody (3-B6) [NBP2-66916] - Staining of F9 cells using anti-Kidins220 Mouse mAb.

Immunohistochemistry-Paraffin: KIDINS220 Antibody (3-B6) [NBP2-66916] - Analysis of paraffin- embedded mouse brain tissue using anti-Kidins220 Mouse mAb.

Western Blot: KIDINS220 Antibody (3-B6) [NBP2-66916] - Analysis on recombinant protein using anti-Kidins220 Mouse mAb.

Product Discontinued

KIDINS220 Antibody (3-B6) Summary

• Immunogen: Recombinant protein with Human KIDINS220 aa 1601-1771 / 1,771. (SwissProt: Q9ULH0 Human; SwissProt: E9Q9B7 Mouse)
• Localization: Cell membrane
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KIDINS220
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:1000-1:10000

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Protein A purified

Alternate Names for KIDINS220 Antibody (3-B6)

• kinase D-interacting substrate, 220kDa

Background

KIDINS220 is a novel protein kinase D-interacting substrate of 220 kDa (Kidins220) is the first identified Protein kinase D (PKD), a serine/threonine kinase. Kidins220 is an integral membrane protein that is expressed in brain and neuroendocrine cells. Kidins220 is phosphorylated by PKD at serine 919 in vivo. Kidins220 has also been shown to function downstream of the Trks and Eph receptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KIDINS220