KCNK1 Overexpression Lysate (Native)

Images

 
Western Blot: KCNK1 Overexpression Lysate (Adult Normal) [NBL1-12176] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for KCNK1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

KCNK1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC205321 and based on accession number NM_002245. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens potassium channel, subfamily K, member 1 (KCNK1), mRNA.
Gene
KCNK1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for KCNK1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for KCNK1 Overexpression Lysate (Native)

  • DPK
  • HOHO1
  • Inward rectifying potassium channel protein TWIK-1
  • K2P1
  • K2p1.1
  • KCNO1
  • Potassium channel KCNO1
  • potassium channel subfamily K member 1
  • potassium channel, subfamily K, member 1
  • potassium inwardly-rectifying channel, subfamily K, member 1
  • TWIK-1
  • TWIK1HOHO

Background

This gene encodes one of the members of the superfamily of potassium channel proteins containing two pore-forming P domains. The product of this gene has not been shown to be a functional channel, however, it may require other non-pore-forming proteins for activity. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-42202
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC
NB110-41535
Species: Hu, Mu, Bv
Applications: ICC/IF (-), IHC (-), WB
NBP2-34063
Species: Hu
Applications: IHC, IHC-P
NBP2-32432
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-04360
Species: Hu
NBP1-69621
Species: Hu
Applications: WB
NBP1-81571
Species: Hu
Applications: IHC, IHC-P
NBP1-80271
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP1-49045
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
H00027161-M01
Species: Hu, Mu, Rt
Applications: WB, ChIP, ELISA, ICC/IF, IHC, IHC-P, IP, RNAi
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NB100-1869
Species: Hu, Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Flow-IC
NBP2-57882
Species: Hu
Applications: ICC/IF
NB110-74682
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
AF467
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
H00003748-M01
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF
NBP2-12900
Species: Mu
Applications: WB, ICC/IF, IHC, IHC-P

Publications for KCNK1 Lysate (NBL1-12176) (0)

There are no publications for KCNK1 Lysate (NBL1-12176).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for KCNK1 Lysate (NBL1-12176) (0)

There are no reviews for KCNK1 Lysate (NBL1-12176). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for KCNK1 Lysate (NBL1-12176) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional KCNK1 Products

Bioinformatics Tool for KCNK1 Lysate (NBL1-12176)

Discover related pathways, diseases and genes to KCNK1 Lysate (NBL1-12176). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for KCNK1 Lysate (NBL1-12176)

Discover more about diseases related to KCNK1 Lysate (NBL1-12176).
 

Pathways for KCNK1 Lysate (NBL1-12176)

View related products by pathway.

PTMs for KCNK1 Lysate (NBL1-12176)

Learn more about PTMs related to KCNK1 Lysate (NBL1-12176).

Blogs on KCNK1

There are no specific blogs for KCNK1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

KCNK1 Antibody
NBP1-84987

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our KCNK1 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol KCNK1