KCNK1 Antibody

Western Blot: KCNK1 Antibody [NBP2-76940] - Western blot analysis of KCNK1 on different cell lysate using anti-KCNK1 antibody at 1/500 dilution.Positive control:Lane1: Human placentaLane2: Human liver

Immunohistochemistry: KCNK1 Antibody [NBP2-76940] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-KCNK1 antibody. Counter stained with hematoxylin.

Flow Cytometry: KCNK1 Antibody [NBP2-76940] - Flow cytometric analysis of SH-SY-5Y cells with KCNK1 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunohistochemistry: KCNK1 Antibody [NBP2-76940] - Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-KCNK1 antibody. Counter stained with hematoxylin.

Immunohistochemistry: KCNK1 Antibody [NBP2-76940] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-KCNK1 antibody. Counter stained with hematoxylin.

Immunohistochemistry: KCNK1 Antibody [NBP2-76940] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-KCNK1 antibody. Counter stained with hematoxylin.

Immunohistochemistry: KCNK1 Antibody [NBP2-76940] - Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-KCNK1 antibody. Counter stained with hematoxylin.

Product Discontinued

KCNK1 Antibody Summary

• Immunogen: Synthetic peptide within human KCNK1 aa 40-100. (SwissProt: O00180 Human; SwissProt: O08581 Mouse; SwissProt: Q9Z2T2 Rat)
• Localization: Cell junction. Cell membrane. Cell projection. Cytoplasmic vesicle. Endosome. Membrane. Synapse.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KCNK1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for KCNK1 Antibody

• DPK
• HOHO1
• Inward rectifying potassium channel protein TWIK-1
• K2P1
• K2p1.1
• KCNK1
• KCNO1
• Potassium channel KCNO1
• potassium channel subfamily K member 1
• potassium channel, subfamily K, member 1
• potassium inwardly-rectifying channel, subfamily K, member 1
• TWIK1
• TWIK-1
• TWIK1HOHO

Background

K+ channels are divided into three subclasses, reflecting the number of transmembrane segments (TMS), which are designated 6TMS, 4TMS and 2TMS. Members of the 4TMS class contain two distinct pore regions, and include TASK, TREK, TRAAK and TWIK. TWIK-1 mRNA is expressed abundantly in brain and at lower levels in lung, kidney and skeletal muscle. The molecular weight of TWIK-1 in mouse brain is 40 kDa under reducing conditions. TWIK-2 shares low sequence homology with other mammalian family group members, and only 34% homology with TWIK-1. Human TWIK-2 is expressed in pancreas, placenta and heart, while mouse TWIK-2 is expressed in liver. TWIK-2 is inhibited by intracellular, but not extracellular, acidification.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KCNK1