Recombinant Human KCNJ10 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, PA

Order Details

Recombinant Human KCNJ10 Protein Summary

Description
Recombinant protein with GST-tag at N-terminal corresponding to the amino acids 276 - 379 of Human KCNJ10 partial ORF

Source: Wheat Germ (in vitro)

Amino Acid Sequence:DFELVLILSGTVESTSATCQVRTSYLPEEILWGYEFTPAISLSASGKYIADFSLFDQVVKVASPSGLRDSTVRYGDPEKLKLEESLREQAEKEGSALSVRISNV

Details of Functionality
This protein is not active and should not be used for experiments requiring activity.
Protein/Peptide Type
Partial Recombinant Protein
Gene
KCNJ10
Purity
>80%, by SDS-PAGE

Applications/Dilutions

Application Notes
This protein has not been tested for any functionality. Product may contain endotoxins and is not suitable for use with live cells.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Purity
>80%, by SDS-PAGE

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human KCNJ10 Protein

  • ATP-dependent inwardly rectifying potassium channel Kir4.1
  • ATP-sensitive inward rectifier potassium channel 10
  • BIRK-10
  • glial ATP-dependent inwardly rectifying potassium channel KIR4.1
  • Inward rectifier K(+) channel Kir1.2
  • inward rectifier K+ channel KIR1.2
  • KCNJ13-PEN
  • KIR1.2
  • KIR4.1
  • Potassium channel, inwardly rectifying subfamily J member 10
  • potassium inwardly-rectifying channel, subfamily J, member 10
  • SESAME

Background

KCNJ10 - potassium inwardly-rectifying channel, subfamily J, member 10

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Publications for KCNJ10 Partial Recombinant Protein (H00003766-Q01) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for KCNJ10 Partial Recombinant Protein (H00003766-Q01). (Showing 1 - 1 of 1 FAQ).

  1. I would like to know which specific peptide sequence are detected by the antibody? It is only stated in the data sheet that it reacts against a part of the C-terminal part of KIR4.1. Also, do you know if this antibody or any of your other antibodies could be utilized as an intracellular staining of cells for flow cytometry? Furthermore, I have seen in the data sheet for the synthetic peptide that it is not suitable for use with live cells, but is this an absolute? If this is the case, would it possible to buy a similar peptide which could be used with live cells, and if so at which prize/amount? Lastly, are the peptide in a frozen liquid form (or solid powder) with out trace of azide? When it comes to the cell lysate I'm wondering if this could be utilized with live cells or if the RIPA buffer which it is dissolved in makes this very toxic? If the last is the case, is it possible to provide the lysate in another buffer which are appropriate for live cells? Lastly, I wondered if the transfected HEK293T cells are also sold for culture?
    • This partial recombinant protein is produced by a company in Taiwan called Abnova. We distribute their products in addition to selling our own. They have confirmed that this protein has not been tested with live cells, so we do not know whether it would be suitable or not, and that it is supplied frozen, in an azide-free buffer. Unfortunately, an alternative protein/peptide of KCNJ10 is not available. I think this product is supplied frozen not lyophilized, and the buffer is 50 mM Tris-HCI, 10 mM reduced glutathione, pH 8.0.

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Bioinformatics Tool for KCNJ10 Partial Recombinant Protein (H00003766-Q01)

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Bioinformatics

Gene Symbol KCNJ10