Human KCNJ10 - Ready-To-Use ELISA Kit (Colorimetric)

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ELISA: Human KCNJ10 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39435] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Tissue homogenates, cell lysates and other biological fluids
Standard Curve Range
1.56 - 100 ng/mL (example only; lot dependent)
Sensitivity
0.64 ng/mL (example only; lot dependent)

Order Details

Human KCNJ10 - Ready-To-Use ELISA Kit (Colorimetric) Summary

Description
The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
Assay Length: 3 hours
Standard Curve Range
1.56 - 100 ng/mL (example only; lot dependent)
Sensitivity
0.64 ng/mL (example only; lot dependent)
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12 (example only; lot dependent)
Intra-Assay
%CV < 10 (example only; lot dependent)
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
KCNJ10

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Detection Solution A
  2. Detection Solution B
  3. Instruction manual
  4. Plate sealer for 96 wells
  5. Pre-coated 96T strip plate
  6. Standard Diluent
  7. Standard
  8. Stop Solution
  9. TMB Substrate
  10. Wash Buffer (30 x concentrate)

Alternate Names for Human KCNJ10 - Ready-To-Use ELISA Kit (Colorimetric)

  • ATP-dependent inwardly rectifying potassium channel Kir4.1
  • ATP-sensitive inward rectifier potassium channel 10
  • BIRK-10
  • glial ATP-dependent inwardly rectifying potassium channel KIR4.1
  • Inward rectifier K(+) channel Kir1.2
  • inward rectifier K+ channel KIR1.2
  • KCNJ10
  • KCNJ13-PEN
  • KIR1.2
  • Kir4.1
  • Potassium channel, inwardly rectifying subfamily J member 10
  • potassium inwardly-rectifying channel, subfamily J, member 10
  • SESAME

Background

KCNJ10 encodes a member of the inward rectifier-type potassium channel family, characterized by having a greater tendency to allow potassium to flow into, rather than out of, a cell. The encoded protein may form a heterodimer with another potassium channel protein and may be responsible for the potassium buffering action of glial cells in the brain. Mutations in this gene have been associated with seizure susceptibility of common idiopathic generalized epilepsy syndromes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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FAQs for KCNJ10 ELISA Kit (NBP3-39435). (Showing 1 - 1 of 1 FAQ).

  1. What is the difference between the Quantikine and Duoset ELISA kits?
    • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

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Bioinformatics

Gene Symbol KCNJ10