KCC3/SLC12A6 Antibody

Western Blot: KCC3/SLC12A6 Antibody [NBP1-06044] - (0.1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: KCC3/SLC12A6 Antibody [NBP1-06044] - (2.5ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

KCC3/SLC12A6 Antibody Summary

• Description: Novus Biologicals Goat KCC3/SLC12A6 Antibody (NBP1-06044) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-KCC3/SLC12A6 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence SRHIDVCSKTKE corresponding to internal region according to NP_001035959.1, NP_001035960.1, NP_001035961.1, NP_001035962.1, NP_005126.1, NP_598408.1.
• Epitope: SRHIDVCSKTKE
• Specificity: This antibody is expected to recognise all 6 reported isofoms (NP_598408.1; NP_005126.1; NP_001035959.1; NP_001035960.1; NP_001035961.1; NP_001035962.1; );
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SLC12A6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2 - 4 ug/ml
  • Immunohistochemistry-Paraffin 2 - 4 ug/ml
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 120 kDa band observed in human heart and kidney lysates (calculated MW ranging 121-128 kDa band according to NP_598408.1; NP_005126.1; NP_001035959.1; NP_001035960.1; NP_001035961.1; NP_001035962.1). IHC-P: Human heart shows staining of myocardial fibrils in transverse section.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for KCC3/SLC12A6 Antibody

• ACCPN
• agenesis of corpus callosum and peripheral neuropathy (Andermann syndrome)
• DKFZp434D2135
• EC 1.2.4.1
• EC 6.1.1.3
• Electroneutral potassium-chloride cotransporter 3
• KCC3
• KCC3A
• KCC3B
• KCC3potassium chloride cotransporter 3
• K-Cl cotransporter 3
• potassium chloride cotransporter KCC3a-S3
• potassium-chloride transporter-3a
• potassium-chloride transporter-3b
• SLC12A6
• solute carrier family 12 (potassium/chloride transporters), member 6
• solute carrier family 12 member 6

Background

This gene is a member of the K-Cl cotransporter (KCC) family. K-Cl cotransporters are integral membrane proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The proteins encoded by this gene are activated by cell swelling induced by hypotonic conditions. Alternate splicing results in multiple transcript variants encoding different isoforms. Mutations in this gene are associated with agenesis of the corpus callosum with peripheral neuropathy. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KCC3/SLC12A6 Antibody (NBP1-06044)(2)

Publications using NBP1-06044

• Hishikawa A, Hayashi K, Kubo A et al. DNA repair factor KAT5 prevents ischemic acute kidney injury through glomerular filtration regulation iScience 2021-12-17 [PMID: 34877495]
• Simard CF, Bergeron MJ, Frenette-Cotton R et al. Homooligomeric and heterooligomeric associations between K+-Cl- cotransporter isoforms and between K+-Cl- and Na+-K+-Cl- cotransporters. J Biol Chem 2007-06-22 [PMID: 17462999]

Bioinformatics

• Gene Symbol: SLC12A6
• Entrez:
  • 9990(Human)
• Uniprot:
  • NP_001035959.1(Human)