Kallikrein 6/Neurosin Antibody - BSA Free

Western Blot: Kallikrein 6/Neurosin Antibody [NBP1-06497] - Human salivary lysates.

Immunocytochemistry/Immunofluorescence: Kallikrein 6/Neurosin Antibody [NBP1-06497] - Kallikrein 6 antibody was tested in Hek293 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Kallikrein 6/Neurosin Antibody - BSA Free Summary

• Immunogen: Full length human Kallikrein 6 protein expressed in E. coli. [Swiss-Prot# Q92876]
• Localization: Cytoplasm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KLK6
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:20
  • Western Blot 1:1000
• Application Notes: This Kallikrein 6 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen at ~38kDa due to glycosylation. In ICC/IF cytoplasmic staining was observed. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Kallikrein 6/Neurosin Overexpression Lysate

Reactivity Notes

Immunogen sequence has 85% identity with canine, 82% identity with equine and 80% identity with bovine.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.1% Sodium Azide
• Purity: Unpurified

Alternate Names for Kallikrein 6/Neurosin Antibody - BSA Free

• Bssp
• EC 3.4.21
• EC 3.4.21.-
• hK6
• kallikrein 6 (neurosin, zyme)
• Kallikrein 6
• kallikrein-6
• kallikrein-related peptidase 6
• KLK6
• Klk7
• Neurosin
• Protease M
• protease, serine, 18
• PRSS18
• PRSS9
• PRSS9MGC9355
• Serine protease 18
• Serine protease 9
• SP59
• Zyme

Background

Kallikrein 6 (KLK6 or kallikrein-related peptidase 6) is a serine protease with trypsin-like properties and belongs to family of KLKs that serves multiple physiological functions including blood pressure regulation, semen liquefaction, skin desquamation etc. and have also been known to implicate in several pathological processes such as tumorigenesis/metastasis, angiogenesis, neurological diseases etc. KLK6 shows activity against amyloid precursor protein, myelin basic protein, gelatin, casein and extracellular matrix proteins including fibronectin, laminin, vitronectin as well as collagen. KLK6 exerts degradation mediated prevention of alpha-synuclein polymerization, suggesting its role in synucleinopathies such as Parkinson disease. KLK6 has also been proposed to involve in regulation of axon outgrowth following spinal cord injury and in pathways including inflammation, receptor activation and apoptosis regulation. Expressed at highest levels brain among other normal tissues, KLK6 has been extensively studied in cancer as well as neurodegenerative diseases wherein it has emerged as a novel biomarkers for prognosis, diagnosis and therapeutic intervention.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KLK6
• Entrez:
  • 5653(Human)
• Uniprot:
  • Q92876()