Kallikrein 6/Neurosin Overexpression Lysate Summary
Description |
Transient overexpression lysate of kallikrein-related peptidase 6 (KLK6), transcript variant A The lysate was created in HEK293T cells, using Plasmid ID RC218647 and based on accession number NM_002774. The protein contains a C-MYC/DDK Tag. |
Gene |
KLK6 |
Applications/Dilutions
Dilutions |
|
Application Notes |
This product is intended for use as a positive control in Western Blot.
You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). |
Theoretical MW |
25.1 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at -80C. Avoid freeze-thaw cycles. |
Buffer |
RIPA buffer |
Lysate Details for Array
Notes
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for Kallikrein 6/Neurosin Overexpression Lysate
Background
Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. The encoded enzyme is regulated by steroid hormones. In tissue culture, the enzyme has been found to generate amyloidogenic fragments from the amyloid precursor protein, suggesting a potential for involvement in Alzheimer's disease. Multiple alternatively spliced transcript variants that encode different isoforms have been identified for this gene. [provided by RefSeq]. Transcript Variant: This variant (A), also called the classical form, is the most frequently occurring transcript. Variants A and B encode the same isoform (A). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are
guaranteed for 6 months from date of receipt.
Customers Who Viewed This Item Also Viewed...
Species: Hu
Applications: ELISA
Species: Hu
Applications: EnzAct
Species: Hu
Applications: IHC, IP, Neut, WB
Species: Hu
Applications: EnzAct
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
Species: Mu
Applications: IHC, WB
Species: Hu
Applications: EnzAct
Species: Hu
Applications: IHC, IHC-P, PEP-ELISA, WB
Species: Hu
Applications: Simple Western, WB
Species: Hu, Mu
Applications: ChIP, ELISA, IHC, IHC-P, WB
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, KD, WB
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu
Applications: IHC, IP, WB
Species: Hu
Applications: IHC, IP, Neut, WB
Species: Hu, Mu
Applications: IHC, IP, WB
Species: Bt, Bv, Ca, Eq, Hu, Pm, Mu, Rb
Applications: IHC, IHC-P
Species: Hu
Applications: IHC, WB
Species: Hu
Applications: DirELISA, IP, WB
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB
Publications for Kallikrein 6/Neurosin Lysate (NBL1-12354) (0)
There are no publications for Kallikrein 6/Neurosin Lysate (NBL1-12354).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for Kallikrein 6/Neurosin Lysate (NBL1-12354) (0)
There are no reviews for Kallikrein 6/Neurosin Lysate (NBL1-12354).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Video Protocols
FAQs for Kallikrein 6/Neurosin Lysate (NBL1-12354) (0)
Additional Kallikrein 6/Neurosin Products
Bioinformatics Tool for Kallikrein 6/Neurosin Lysate (NBL1-12354)
Discover related pathways, diseases and genes to Kallikrein 6/Neurosin Lysate (NBL1-12354). Need help?
Read the
Bioinformatics Tool Guide for instructions on using this tool.
Diseases for Kallikrein 6/Neurosin Lysate (NBL1-12354)
Discover more about diseases related to Kallikrein 6/Neurosin Lysate (NBL1-12354).
| | Pathways for Kallikrein 6/Neurosin Lysate (NBL1-12354)
View related products by pathway.
|
PTMs for Kallikrein 6/Neurosin Lysate (NBL1-12354)
Learn more about PTMs related to Kallikrein 6/Neurosin Lysate (NBL1-12354).
| | Research Areas for Kallikrein 6/Neurosin Lysate (NBL1-12354)
Find related products by research area.
|
Blogs on Kallikrein 6/Neurosin