Kallikrein 3/PSA Antibody (A67-B/E3) [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: Flow, IHC, CyTOF-ready
• Clone: A67-B/E3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 405

Kallikrein 3/PSA Antibody (A67-B/E3) [Alexa Fluor® 405] Summary

• Immunogen: Kallikrein 3/PSA (p30) from human sperm plasma (Uniprot: P07288)
• Localization: Cytoplasmic
• Specificity: Recognizes a single protein of 33-34kDa, identified as the prostate specific antigen (PSA). This monoclonal antibody is highly specific to PSA and stains prostatic secretory and ductal epithelium in both normal and neoplastic tissues. PSA is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate epithelium, and abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia. This monoclonal antibody makes an excellent pair with monoclonal antibody 1A7G6B6 for PSA tests.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: KLK3
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Use in Flow Cytometry reported in scientific literature (PMID: 33364756).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Kallikrein 3/PSA Antibody (A67-B/E3) [Alexa Fluor® 405]

• APS seminin
• EC 3.4.21
• EC 3.4.21.77
• Gamma-seminoprotein
• hK3
• Kallikrein 3
• kallikrein 3, (prostate specific antigen)
• Kallikrein-3
• kallikrein-related peptidase 3
• KLK2A1
• KLK3
• P-30 antigen
• prostate specific antigen
• prostate-specific antigen
• PSA semenogelase
• PSA
• Semenogelase
• Seminin

Background

PSA is a chymotrypsin-like serine protease (kallikrein family) produced by the prostate epithelium, and abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liverderived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Kallikrein 3/PSA Antibody (NBP2-34549AF405)(1)

Publication using NBP2-34549AF405

• Pang B, Zhu Y, Ni J et al Quality Assessment and Comparison of Plasma-Derived Extracellular Vesicles Separated by Three Commercial Kits for Prostate Cancer Diagnosis Int J Nanomedicine 2020-12-28 [PMID: 33364756] (FLOW, Human)
  
  Details:
  Citation using the Alexa Fluor 405 version of this antibody.

Bioinformatics

• Gene Symbol: KLK3