JWA Antibody

Western Blot: JWA Antibody [NB100-1105] - Staining of Human Brain lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: JWA Antibody [NB100-1105] - Staining of Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: JWA Antibody [NB100-1105] - Staining of Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Discontinued

JWA Antibody Summary

• Immunogen: Peptide with sequence C-NRLTDYISKVKE corresponding to C-Terminus according to NP_006398.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ARL6IP5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3.75 ug/ml
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 20 kDa band observed in human brain lysates (predicted MW of 21.6 kDa band according to NP_006398.1). IHC: Paraffin embedded Human Tonsil and Small Intestine.
• Control:

  Human Liver Whole Tissue Lysate (Adult Whole Normal)
  Human Brain Frontal Lobe Membrane Tissue Lysate (Adult Membrane Normal)

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:19208635).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for JWA Antibody

• ADP-ribosylation factor-like protein 6-interacting protein 5
• ADP-ribosylation-like factor 6 interacting protein 5
• Aip-5
• ARL-6-interacting protein 5
• Cytoskeleton-related vitamin A-responsive protein
• Dermal papilla-derived protein 11
• DERP11addicsin
• Glutamate transporter EAAC1-interacting protein
• glutamate transporter EEAC1-associated protein
• GTRAP3-18aip-5
• hp22
• HSPC127
• JM5
• jmx
• JWAcytoskeleton related vitamin A responsive protein
• PRA1 domain family 3
• PRA1 family protein 3
• PRA2
• PRAF3dermal papilla derived protein 11
• Prenylated Rab acceptor protein 2
• Protein JWa
• putative MAPK activating protein PM27
• Putative MAPK-activating protein PM27

Background

The function of this protein is unknown but a similar protein in rats may play a role in the regulation of cell differentiation. The rat protein binds and inhibits the cell membrane glutamate transporter EAAC1. The expression of the rat gene is upregulated by retinoic acid, which results in a specific reduction in EAAC1-mediated glutamate transport.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for JWA Antibody (NB100-1105)(2)

Publications using NB100-1105

• Wang S, Gong Z, Chen R et al. JWA regulates XRCC1 and functions as a novel base excision repair protein in oxidative-stress-induced DNA single-strand breaks. Nucleic Acids Res. 2009-04-01 [PMID: 19208635] (WB, Mouse)
  
  Details:
  WB (mouse NIH-3T3 cells), Figs. 1B; 2B,C; 3E,F; 5E.
• Lin CI, Orlov I, Ruggiero AM et al. Modulation of the neuronal glutamate transporter EAAC1 by the interacting protein GTRAP3-18. Nature 2001-03-01 [PMID: 11242046]

Bioinformatics

• Gene Symbol: ARL6IP5
• Entrez:
  • 10550(Human)
• Uniprot:
  • NP_006398.(Human)