JWA Antibody

Images

 
Western Blot: JWA Antibody [NB100-1105] - Staining of Human Brain lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: JWA Antibody [NB100-1105] - Staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: JWA Antibody [NB100-1105] - Staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

JWA Antibody Summary

Immunogen
Peptide with sequence C-NRLTDYISKVKE corresponding to C-Terminus according to NP_006398.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ARL6IP5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.3 ug/ml
  • Immunohistochemistry 3.75 ug/ml
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 20 kDa band observed in human brain lysates (predicted MW of 21.6 kDa band according to NP_006398.1). IHC: Paraffin embedded Human Tonsil and Small Intestine.
Positive Control
Liver Lysate (NB820-59232)
Brain Frontal Lobe Lysate (NB820-60576)
Publications
Read Publications using
NB100-1105 in the following applications:

  • WB
    1 publication

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for JWA Antibody

  • ADP-ribosylation factor-like protein 6-interacting protein 5
  • ADP-ribosylation-like factor 6 interacting protein 5
  • Aip-5
  • ARL-6-interacting protein 5
  • Cytoskeleton-related vitamin A-responsive protein
  • Dermal papilla-derived protein 11
  • DERP11addicsin
  • Glutamate transporter EAAC1-interacting protein
  • glutamate transporter EEAC1-associated protein
  • GTRAP3-18aip-5
  • hp22
  • HSPC127
  • JM5
  • jmx
  • JWAcytoskeleton related vitamin A responsive protein
  • PRA1 domain family 3
  • PRA1 family protein 3
  • PRA2
  • PRAF3dermal papilla derived protein 11
  • Prenylated Rab acceptor protein 2
  • Protein JWa
  • putative MAPK activating protein PM27
  • Putative MAPK-activating protein PM27

Background

The function of this protein is unknown but a similar protein in rats may play a role in the regulation of cell differentiation. The rat protein binds and inhibits the cell membrane glutamate transporter EAAC1. The expression of the rat gene is upregulated by retinoic acid, which results in a specific reduction in EAAC1-mediated glutamate transport.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for JWA Antibody (NB100-1105)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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WB
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ARL6IP5
Entrez
Uniprot