JunB/AP-1 Antibody

Images

 
Western Blot: JunB/AP-1 Antibody [NBP1-51950] - Analysis of JunB in Human Breast Cancer lysate (35ug protein in RIPA buffer) using NBP1-51950 at 0.3 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

JunB/AP-1 Antibody Summary

Immunogen
Peptide with sequence C-TLKAENAGLSSTAG corresponding to internal region according to NP_002220.1.
Epitope
C-TLKAENAGLSSTAG
Specificity
This antibody may also cross-react with human, mouse, rat, cow, dog JUN (GeneID: 3725, 16476, 24516, 280831, 609429).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
JUNB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Approx. 35 kDa band observed in human breast cancer lysates (calculated MW of 35.9 kDa band according to NP_002220.1).
Publications
Read Publication using NBP1-51950.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Bovine, Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for JunB/AP-1 Antibody

  • activator protein 1
  • AP-1
  • jun B proto-oncogene
  • JunB
  • transcription factor jun-B

Background

The cJun proto-oncogene was first identified as the cellular homolog of the avian sarcoma virus vjun oncogene. JunB and JunD have been shown to be almost identical to cJun in their C terminal regions, which are involved in dimerization and DNA binding, whereas their N terminal domains, which are involved in transcriptional activation, diverge.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for JunB/AP-1 Antibody (NBP1-51950)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol JUNB
Entrez
Uniprot