| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08390 | |||
HAF016). A specific band for JunB was detected at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Sheep Anti-Human JunB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4456) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for JunB was detected at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4456-SP | |||
HAF007). A specific band was detected for JunB at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and PC-3 human prostate cancer cell line. PVDF Membrane was probed with 1 µg/mL of Human JunB Monoclonal Antibody (Catalog # MAB4456) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for JunB at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB4456-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08391 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26185-50ul | |||
![Immunocytochemistry/Immunofluorescence: JunB/AP-1 Antibody [NBP1-89544] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/JunB-AP-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89544-img0011.jpg "Immunocytochemistry/Immunofluorescence: JunB/AP-1 Antibody [NBP1-89544] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: JunB/AP-1 Antibody [NBP1-89544] - Staining in human fallopian tube and skeletal muscle tissues . Corresponding JUNB RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/JunB-AP-1-Antibody-Immunohistochemistry-Paraffin-NBP1-89544-img0016.jpg)
![Immunohistochemistry-Paraffin: JunB/AP-1 Antibody [NBP1-89544] - Staining of human fallopian tube shows moderate to strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/JunB-AP-1-Antibody-Immunohistochemistry-Paraffin-NBP1-89544-img0013.jpg)
![Immunohistochemistry-Paraffin: JunB/AP-1 Antibody [NBP1-89544] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.](http://images.novusbio.com/fullsize/JunB-AP-1-Antibody-Immunohistochemistry-Paraffin-NBP1-89544-img0014.jpg)
![Immunohistochemistry-Paraffin: JunB/AP-1 Antibody [NBP1-89544] - Staining of human colon shows moderate to strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/JunB-AP-1-Antibody-Immunohistochemistry-Paraffin-NBP1-89544-img0012.jpg)
![Immunohistochemistry-Paraffin: JunB/AP-1 Antibody [NBP1-89544] - Staining of human testis shows moderate nuclear positivity in a subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/JunB-AP-1-Antibody-Immunohistochemistry-Paraffin-NBP1-89544-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: JunB/AP-1 Antibody (PCRP-JUNB-3G11) [NBP3-07578] - Immunofluorescence Analysis of HeLa cells using JunB/AP-1 Mouse Monoclonal Antibody (PCRP-JUNB-3G11) followed by goat anti- Mouse IgG-CF488 (green). Phalloidin counterstain.](http://images.novusbio.com/fullsize/JunB-AP-1-Antibody-PCRP-JUNB-3G11-Immunocytochemistry-Immunofluorescence-NBP3-07578-img0003.jpg "Immunocytochemistry/Immunofluorescence: JunB/AP-1 Antibody (PCRP-JUNB-3G11) [NBP3-07578] - Immunofluorescence Analysis of HeLa cells using JunB/AP-1 Mouse Monoclonal Antibody (PCRP-JUNB-3G11) followed by goat anti- Mouse IgG-CF488 (green). Phalloidin counterstain.")
![Flow Cytometry: JunB/AP-1 Antibody (PCRP-JUNB-3G11) [NBP3-07578] - Flow Cytometric Analysis of PFA-fixed HeLa cells. JunB/AP-1 Mouse Monoclonal Antibody (PCRP-JUNB-3G11) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).](http://images.novusbio.com/fullsize/JunB-AP-1-Antibody-PCRP-JUNB-3G11-Flow-Cytometry-NBP3-07578-img0002.jpg)
![Protein Array: JunB/AP-1 Antibody (PCRP-JUNB-3G11) [NBP3-07578] - Analysis of Protein Array containing more than 19,000 full-length human proteins using JunB/AP-1 Mouse Monoclonal Antibody (PCRP-JunB/AP-1-3G11).](http://images.novusbio.com/fullsize/JunB-AP-1-Antibody-PCRP-JUNB-3G11-Protein-Array-NBP3-07578-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: JunB/AP-1 Antibody (PCRP-JUNB-3G2) [NBP3-07579] - Immunofluorescence Analysis of PFA-HeLa cells. JunB/AP-1 Mouse Monoclonal Antibody (PCRP-JunB/AP-1-3G2) followed by goat anti- Mouse IgG-CF488 (green). Phalloidin counterstain (red).](http://images.novusbio.com/fullsize/JunB-AP-1-Antibody-PCRP-JUNB-3G2-Immunocytochemistry-Immunofluorescence-NBP3-07579-img0003.jpg "Immunocytochemistry/Immunofluorescence: JunB/AP-1 Antibody (PCRP-JUNB-3G2) [NBP3-07579] - Immunofluorescence Analysis of PFA-HeLa cells. JunB/AP-1 Mouse Monoclonal Antibody (PCRP-JunB/AP-1-3G2) followed by goat anti- Mouse IgG-CF488 (green). Phalloidin counterstain (red).")
![Flow Cytometry: JunB/AP-1 Antibody (PCRP-JUNB-3G2) [NBP3-07579] - Flow Cytometric Analysis of PFA-fixed HeLa cells. JunB/AP-1 Mouse Monoclonal Antibody (PCRP-JunB/AP-1-3G2) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).](http://images.novusbio.com/fullsize/JunB-AP-1-Antibody-PCRP-JUNB-3G2-Flow-Cytometry-NBP3-07579-img0002.jpg)
![Protein Array: JunB/AP-1 Antibody (PCRP-JUNB-3G2) [NBP3-07579] - Analysis of Protein Array containing more than 19,000 full-length human proteins using JunB/AP-1 Mouse Monoclonal Antibody (PCRP-JunB/AP-1-3G2).](http://images.novusbio.com/fullsize/JunB-AP-1-Antibody-PCRP-JUNB-3G2-Protein-Array-NBP3-07579-img0001.jpg)
![Western Blot: JunB/AP-1 Antibody (3U10K1) [NBP3-16491] - Western blot analysis of extracts of BxPC-3 cells, using JunB/AP-1 Rabbit mAb (NBP3-16491) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/JunB-AP-1-Antibody-3U10K1-Western-Blot-NBP3-16491-img0002.jpg "Western Blot: JunB/AP-1 Antibody (3U10K1) [NBP3-16491] - Western blot analysis of extracts of BxPC-3 cells, using JunB/AP-1 Rabbit mAb (NBP3-16491) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: JunB/AP-1 Antibody (3U10K1) [NBP3-16491] - Immunohistochemistry of paraffin-embedded human esophageal using JunB/AP-1 Rabbit mAb (NBP3-16491) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/JunB-AP-1-Antibody-3U10K1-Immunohistochemistry-Paraffin-NBP3-16491-img0001.jpg)
![Immunohistochemistry: JunB/AP-1 Antibody (5H4) [NBP3-26185] - Image of JunB/AP-1 Antibody (5H4) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26185_rabbit-junb-ap-1-mab-5h4-262202415475642.jpg "Immunohistochemistry: JunB/AP-1 Antibody (5H4) [NBP3-26185] - Image of JunB/AP-1 Antibody (5H4) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunocytochemistry/Immunofluorescence: JunB/AP-1 Antibody (5H4) [NBP3-26185] - Staining of MCF7 Cells with JunB/AP-1 Antibody (5H4) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26185_rabbit-junb-ap-1-mab-5h4-282202412493644.jpg)
![Western Blot: JunB/AP-1 Antibody [NBP1-51950] - Analysis of JunB in Human Breast Cancer lysate (35ug protein in RIPA buffer) using NBP1-51950 at 0.3 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/JunB-AP-1-Antibody-Western-Blot-NBP1-51950-img0002.jpg "Western Blot: JunB/AP-1 Antibody [NBP1-51950] - Analysis of JunB in Human Breast Cancer lysate (35ug protein in RIPA buffer) using NBP1-51950 at 0.3 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.")
