JSRP1 Antibody - BSA Free

Western Blot: JSRP1 Antibody [NBP1-81998] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Skeletal muscle tissue

Immunocytochemistry/Immunofluorescence: JSRP1 Antibody [NBP1-81998] - Staining of human cell line RH-30 shows localization to endoplasmic reticulum. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: JSRP1 Antibody [NBP1-81998] - Staining in human skeletal muscle and heart muscle tissues using anti-JSRP1 antibody. Corresponding JSRP1 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: JSRP1 Antibody [NBP1-81998] - Staining of human heart muscle shows low expression as expected.

Immunohistochemistry-Paraffin: JSRP1 Antibody [NBP1-81998] - Staining of human skeletal muscle shows high expression.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

JSRP1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit JSRP1 Antibody - BSA Free (NBP1-81998) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: EAEVRPKIPGSREAAENDEEEPGEATGEAVREDRVTLADRGPKERPRREGKPRKEKPRKEERPKKERPRKEER
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: JSRP1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:5000 - 1:10000
  • Immunohistochemistry-Paraffin 1:5000 - 1:10000
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  JSRP1 Protein (NBP1-81998PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for JSRP1 Antibody - BSA Free

• FLJ32416
• JP45
• JP-45homolog of mouse skeletal muscle sarcoplasmic reticulum protein JP-45
• junctional sarcoplasmic reticulum protein 1,2310032K21Rik
• Junctional-face membrane protein of 45 kDa homolog
• skeletal muscle sarcoplasmic reticulum protein JP-45

Background

The junction between the transverse tubules (T-tubules) and the sarcoplasmic reticulum (SR) of skeletal muscle is called the triad. At the triad, dihydropyridine receptors (DHPR®s) of the T-tubule serve as voltage sensors in excitation-contraction coupling, while ryanodine receptors (RyR®s), the calcium release channels, exist in the membrane of the terminal cisternae of the SR. It is thought that during slow phase depolarization of the T-tubule, a third protein, triadin (95 kDa) transmits electrochemical signals to the SR through direct interaction with both DHPR®s and RyR®s. Another skeletal muscle specific 90 kDa protein has also been localized to the this junction. This protein is phosphorylated by an intrinsic protein kinase in triads suggesting that it may play an important regulatory or structural role in the skeletal muscle triad junction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: JSRP1