JPH1 Antibody - BSA Free

Western Blot: JPH1 Antibody [NBP1-77338] - 293 cell lysate with JPH1 antibody at (A) 1 and (B) 2 ug/ml.

Immunohistochemistry: JPH1 Antibody - BSA Free [NBP1-77338] - Immunohistochemistry of JPH1 in mouse skeletal muscle tissue with JPH1 antibody at 2.5 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

JPH1 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human JPH1. The immunogen is located within amino acids 470 - 520 of JPH1. Amino Acid Squence: SGARLNQDKRSVADE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: JPH1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1-2 ug/ml
• Control Peptide:

  JPH1 Peptide (NBP1-77338PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rabbit (87%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for JPH1 Antibody - BSA Free

• DKFZp762L0313
• JP-1junctophilin type1
• JP1junctophilin-1
• junctophilin 1
• Junctophilin type 1
• mitsugumin72

Background

Junctional complexes between the plasma membrane (PM) and endoplasmic/sarcoplasmic reticulum (ER/SR) are a common feature of all excitable cell types and mediate cross talk between cell surface and intracellular ion channels. Junctophilins (JPs) are important components of the junctional complexes. JPs are composed of a carboxy-terminal hydrophobic segment spanning the ER/SR membrane and a remaining cytoplasmic domain that shows specific affinity for the PM. Four JPs have been identified as tissue-specific subtypes derived from different genes: JPH1 is expressed in skeletal muscle, JPH2 is detected throughout all muscle cell types, and JPH3 and JPH4 are predominantly expressed in the brain and contribute to the subsurface cistern formation in neurons. JPH1 is essential for stabilizing the T-tubule and SR membranes to form junctions and provide an environment for the assembly of receptors such as the ryanodine receptor type 1 (RyR1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: JPH1
• Uniprot:
  • AAI39833(Human)