JNK2 Antibody - BSA Free

Western Blot: JNK2 Antibody [NBP1-30905] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK2 antibody [N1C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: JNK2 Antibody [NBP1-30905] - Analysis of methanol-fixed MCF-7, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: JNK2 Antibody [NBP1-30905] - Paraffin-embedded N87 xenograft, using antibody at 1:100 dilution.

Western Blot: JNK2 Antibody [NBP1-30905] - Sample (30 ug of whole cell lysate) A: MCF-7 10% SDS PAGE; antibody diluted at 1:1000.

Western Blot: JNK2 Antibody [NBP1-30905] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK2 antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, I, Ch
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

JNK2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit JNK2 Antibody - BSA Free (NBP1-30905) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human JNK2. The exact sequence is proprietary.
• Predicted Species: Chicken (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAPK9
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 48 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Reacts with Plutella xylostella.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for JNK2 Antibody - BSA Free

• c-Jun kinase 2
• c-Jun N-terminal kinase 2
• EC 2.7.11
• EC 2.7.11.24
• JNK2
• JNK2A
• JNK2BETA
• JNK2JNK2ALPHA
• JNK-55
• Jun kinase
• MAP kinase 9
• MAPK 9
• MAPK9
• mitogen-activated protein kinase 9
• p54a
• p54aSAPK
• PRKM9JNK2B
• SAPK
• SAPK1 alpha
• Stress-activated protein kinase JNK2

Background

JNK protein kinases are distantly related to mitogen-activated protein kinases (ERKs) and are activated by dual phosphorylation on Tyr and Thr. The JNK protein kinase group includes the 46-kDa isoform JNK1 and the 55-kDa protein kinase JNK2. The activities of both JNK isoforms are markedly increased by exposure of cells to UV radiation (1). JNK becomes activated in vivo in response to pro-inflammatory cytokines or cellular stresses. Its full activation requires the phosphorylation of a threonine and a tyrosine residue in a Thr-Pro-Tyr motif, which can be catalysed by the protein kinases mitogen-activated protein kinase kinase MKK4 and MKK7 (2). JNK2 functions in a cell-type-specific and stimulus-dependent manner, being required for apoptosis of immature thymocytes induced by anti-CD3 antibody but not for apoptosis induced by anti-Fas antibody, UVC or dexamethasone. JNK2 is not required for activation-induced cell death of mature T cells (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: MAPK9