Human JMJD6/PSR ELISA Kit (Colorimetric) Summary
| Description |
Phosphatidyl Serine Ab ELISA Kit is an indirect solid phase enzyme immunoassay for the quantitative measurement of IgG and IgM class autoantibodies against phosphatidyl serine in human serum or plasma. |
| Standard Curve Range |
IgG: 0 - 100 GPL-U/mL
IgM: 0 - 100 MPL-U/mL |
| Sensitivity |
0.5 GPL-U/mL
0.5 MPL-U/mL |
| Assay Type |
Indirect ELISA |
| Inter-Assay |
IgG CV% < 4.5%
IgM CV% < 4.4% |
| Intra-Assay |
IgG CV% < 3%
IgM CV% < 2.5% |
| Sample Volume |
100 uL (prediluted) |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
JMJD6 |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Useful for quantification. |
Packaging, Storage & Formulations
Kit Components
|
Components
|
- Calibrator A-F (0, 6.3, 12.5, 25, 50, 100 GPL-U/mL / 0, 6.3, 12.5, 25, 50, 100MPL-U/mL) containing serum/buffer matrix (PBS, BSA detergent, NaN3 0.09%), yellow.Ready to use.
- Control positive (1) and negative (2), containing phosphatidyl serine antibodies in aserum/buffer matrix (PBS, BSA detergent, NaN3 0.09%), yellow. Ready to use. Theconcentration is specified on the certificate of analysis.
- Divisible microplate consisting of 12 modules of 8 wells each. Ready to use.
- Enzyme conjugate containing anti-human IgG antibodies, HRP labelled; PBS, BSAdetergent, preservative ProClin 300 0.05%, light red. Ready to use.
- Enzyme conjugate containing anti-human IgM antibodies, HRP labelled; PBS, BSAdetergent, preservative ProClin 300 0.05%, light red. Ready to use.
- Sample Buffer P, containing PBS, BSA detergent, preservative NaN3 0.09%, yellow. 5xconc.
- Stop solution (contains acid). Ready to use.
- TMB substrate containing 3,3’,5,5’-Tetramethylbenzidin, colorless. Ready to use.
- Wash solution containing Tris, detergent, preservative NaN3 0.09%. 50x conc.
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Human JMJD6/PSR ELISA Kit (Colorimetric)
Background
JMJD6 encodes a nuclear protein with a JmjC domain. JmjC domain-containing proteins are predicted to function as protein hydroxylases or histone demethylases. This protein was first identified as a putative phosphatidylserine receptor involved in phagocytosis of apoptotic cells; however, subsequent studies have indicated that it does not directly function in the clearance of apoptotic cells, and questioned whether it is a true phosphatidylserine receptor. Multiple transcript variants encoding different isoforms have been found for this gene.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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FAQs for JMJD6/PSR ELISA Kit (KA1277). (Showing 1 - 1 of 1 FAQ).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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