JAM-A Antibody [Unconjugated]

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JAM‑A was detected in immersion fixed MCF‑7 human breast cancer cell line using Goat Anti-Human JAM‑A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1103) at 10 µg/mL for 3 hours at room ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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JAM-A Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human JAM-A
Ser28-Ala242
Accession # Q9Y624
Specificity
Detects human JAM-A in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant mouse JAM-A is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
F11R
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunocytochemistry 5-15 ug/mL
Publications
Read Publications using
AF1103 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for JAM-A Antibody [Unconjugated]

  • CD321 antigen
  • CD321
  • F11 receptor
  • F11R
  • JAM-1
  • JAM1CD321
  • JAMA
  • JAM-A
  • JCAMJAM
  • Junctional adhesion molecule 1Platelet F11 receptor
  • junctional adhesion molecule A
  • PAM-1
  • PAM-1KAT
  • Platelet adhesion molecule 1

Background

The family of junctional adhesion molecules (JAM), comprising at least three members, are type I transmembrane receptors belonging to the immunoglobulin (Ig) superfamily (1, 2). These proteins are localized in the tight junctions between endothelial or epithelial cells. Some family members are also found on blood leukocytes and platelets. Human JAM-A, also known as platelet adhesion molecule 1 (PAM-1) and platelet F11 receptor (3), is predominantly expressed at intercellular junctions of both epithelial cells and endothelial cells (1‑4). It is also expressed on circulating blood cells including neutrophils, monocytes, platelets, erythrocytes and lymphocytes (5). Human JAM-A cDNA predicts a 299 amino acid (aa) residue precursor protein with a putative 27 aa signal peptide, a 210 aa extracellular region containing two Ig‑like V-subset domains, a 24 aa transmembrane domain and a 38 aa cytoplasmic domain. The human and mouse proteins share approximately 67% aa sequence homology. Human JAM-A also shares approximately 35% and 32% aa sequence homology with human JAM-B and JAM-C, respectively. JAM-A exhibits homophilic interactions to regulate tight junction assembly and modulate paracellular permeability. This homophilic interation also mediates platelet aggregation and adhesion to endothelial cells and may play a role in thrombosis (3). JAM-A binds heterotypically with the beta 2 integrin lymphocyte function-associated antigen-1 (LFA-1). This JAM‑A‑LFA‑1 interaction is involved in leukocyte adhesion and transmigration (6). JAM-A has also been shown to bind reovirus attachment protein sigma-1 to permit reovirus infection and signal virus-induced apoptosis (7).

  1. Chavakis, T. et al. (2003) Thromb. Haemost. 89:13.
  2. Aurand-Lions, M. et al. (2001) Blood 98:3699.
  3. Sobocka, M.B. et al. (2000) Blood 95:2600.
  4. Martin-Padura, I. et al. (1998) J. Cell Biol. 142:117.
  5. Williams, L.A. et. al. (1999) Mol. Immunol. 36:1175.
  6. Ostermann, G. et al. (2002) Nature Immunol. 3:151.
  7. Barton, E.S. et al. (2001) Cell 104:441.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for JAM-A Antibody (AF1103)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 6 applications: ELISA Capture, Flow, ICC, IHC, IP, WB.


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ELISA Capture
(1)
Flow
(1)
ICC
(2)
IHC
(1)
IP
(1)
WB
(2)
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Human
(8)
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Showing Publications 1 - 8 of 8.
Publications using AF1103 Applications Species
L Anton, A DeVine, LJ Sierra, AG Brown, MA Elovitz miR-143 and miR-145 disrupt the cervical epithelial barrier through dysregulation of cell adhesion, apoptosis and proliferation Sci Rep, 2017;7(1):3020. 2017 [PMID: 28596604] (ICC,, Human) ICC Human
Williams D, Anastos K, Morgello S, Berman J JAM-A and ALCAM are therapeutic targets to inhibit diapedesis across the BBB of CD14+CD16+ monocytes in HIV-infected individuals. J Leukoc Biol, 2015;97(2):401-12. 2015 [PMID: 25420915] (ELISA Capture,, Human) ELISA Capture Human
Chattopadhyay R, Dyukova E, Singh N, Ohba M, Mobley J, Rao G Vascular endothelial tight junctions and barrier function are disrupted by 15(S)-hydroxyeicosatetraenoic acid partly via protein kinase C epsilon-mediated zona occludens-1 phosphorylation at threonine 770/772. J Biol Chem, 2014;289(6):3148-63. 2014 [PMID: 24338688] (IP,, Human) IP Human
Teo G, Ankrum J, Martinelli R, Boetto S, Simms K, Sciuto T, Dvorak A, Karp J, Carman C Mesenchymal Stem Cells Transmigrate Between and Directly Through Tumor Necrosis Factor-alpha-Activated Endothelial Cells Via Both Leukocyte-Like and Novel Mechanisms. Stem Cells, 2012;30(11):2472-86. 2012 [PMID: 22887987] (ICC,, Human) ICC Human
Haarmann A, Deiss A, Prochaska J, Foerch C, Weksler B, Romero I, Couraud PO, Stoll G, Rieckmann P, Buttmann M Evaluation of soluble junctional adhesion molecule-A as a biomarker of human brain endothelial barrier breakdown. PLoS ONE, 2010;5(10):e13568. 2010 [PMID: 21060661] (WB,, Human) WB Human
Economopoulou M, Hammer J, Wang F, Fariss R, Maminishkis A, Miller SS Expression, localization, and function of junctional adhesion molecule-C (JAM-C) in human retinal pigment epithelium. Invest. Ophthalmol. Vis. Sci., 2009;50(3):1454-63. 2009 [PMID: 19060272] (WB,, Human) WB Human
Babinska A, Azari BM, Salifu MO, Liu R, Jiang XC, Sobocka MB, Boo D, Al Khoury G, Deitch JS, Marmur JD, Ehrlich YH, Kornecki E The F11 receptor (F11R/JAM-A) in atherothrombosis: overexpression of F11R in atherosclerotic plaques. Thromb. Haemost., 2007;97(2):272-81. 2007 [PMID: 17264957] (IHC,, Human) IHC Human
Makino A, Shimojima M, Miyazawa T, Kato K, Tohya Y, Akashi H Junctional adhesion molecule 1 is a functional receptor for feline calicivirus. J. Virol., 2006;80(9):4482-90. 2006 [PMID: 16611908] (Flow,, Human) Flow Human

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Bioinformatics

Gene Symbol F11R
Entrez
Uniprot