JAM-4/IGSF5 Antibody [Biotin]

• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Biotin
• Concentration: LYOPH

JAM-4/IGSF5 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse JAM‑4/IGSF5
  Leu15-Ala139
  Accession # NP_082354
• Specificity: Detects mouse JAM‑4/IGSF5 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant mouse (rm) JAM-A, rmJAM‑B, and rmJAM-C is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: Igsf5
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

JAM-4, also known as IGSF5, is a 93 kDa member of the CTX (Cortical Thymocyte Marker in Xenopus) subfamily of the Ig superfamily. It is a type I transmembrane protein with an N-terminal extracellular V-type and C2 type Ig domain separated by a J segment, a transmembrane domain and a cytoplasmic tail with a PDZ binding motif. JAM-4 is expressed in intestinal, renal, and mammary epithelial cells, spermatogonia, and hematopoietic stem cells. It is a cell adhesion molecule that mediates homophilic interaction. JAM-4 also interacts with the intracellular membrane-associated guanylate kinase inverted 1 (MAGI-1). An additional isoform containing a 100 residue insertion between Gln 125 and Asp 126 has been reported. Over the region used as immunogen, mouse JAM-4 shares 82% and 63% amino acid sequence identity with rat and human JAM-4, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Igsf5