Human Jak1 ELISA Kit (Colorimetric)

ELISA: Human Jak1 ELISA Kit (Colorimetric) [NBP2-80249] - Samples were spiked with high concentrations of Human Jak1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Jak1 ELISA Kit (Colorimetric) [NBP2-80249] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.156-10 ng/mL
• Sensitivity: 0.094 ng/mL

Human Jak1 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human JAK1 in samples. No significant cross-reactivity or interference between Human JAK1 and analogues was observed.
• Standard Curve Range: 0.156-10 ng/mL
• Sensitivity: 0.094 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.96%
• Intra-Assay: CV% < 5.22%
• Spike Recovery: 86-105%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: JAK1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Jak1 ELISA Kit (Colorimetric)

• EC 2.7.10
• Jak1
• JAK-1
• JAK1Atyrosine-protein kinase JAK1
• JAK1B
• Janus kinase 1EC 2.7.10.2
• JTK3

Background

Janus kinase 1 (JAK1), is a member of a new class of protein-tyrosine kinases (PTK) characterised by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The second phosphotransferase domain bears all the hallmarks of a protein kinase, although its structure differs significantly from that of the PTK and threonine/serine kinase family members. JAK1 is a large, widely expressed membrane-associated phosphoprotein. It is involved in the interferon-alpha/beta and -gamma signal transduction pathways. The reciprocal interdependence between JAK1 and TYK2 activities in the interferon-alpha pathway, and between JAK1 and JAK2 in the interferon-gamma pathway, may reflect a requirement for these kinases in the correct assembly of interferon recpeptor complexes. These kinases couple cytokine ligand binding to tyrosine phosphorylation of various known signalling proteins and a unique family of transcription factors termed the signal transducers and activators of transcription, or STATs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Jak1 ELISA Kit (NBP2-80249)(1)

Publication using NBP2-80249

• Galecka M, Szemraj J, Su KP et al. Is the JAK-STAT Signaling Pathway Involved in the Pathogenesis of Depression? Journal of clinical medicine 2022-04-06 [PMID: 35407663] (ELISA)

FAQs for Jak1 ELISA Kit (NBP2-80249). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: JAK1
• Uniprot:
  • P23458()
  • P23458()