Jak1 Antibody

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Western Blot: Jak1 Antibody [NBP1-31815] - JAK1 antibody detects JAK1 protein analysis A. 30 ug GL261 whole cell lysate/extract B. 30 ug C8D30 whole cell lysate/extract C. 30 ug NIH-3T3 whole cell lysate/extract ...read more
Immunocytochemistry/ Immunofluorescence: JAK1 Antibody [NBP1-31815] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Western Blot: JAK1 Antibody [NBP1-31815] - Sample (30 ug of whole cell lysate) A: Hep G2 5% SDS PAGE; antibody diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Ch, RMSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
5.94 mg/ml

Order Details

Jak1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human JAK1. The exact sequence is proprietary.
Localization
Membrane
Predicted Species
Rat (98%), Porcine (100%), Rhesus Macaque (100%), Bovine (99%), Chicken (93%), Canine (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
JAK1
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Theoretical MW
133 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (85%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
5.94 mg/ml
Purity
Protein A purified

Alternate Names for Jak1 Antibody

  • EC 2.7.10
  • Jak1
  • JAK-1
  • JAK1Atyrosine-protein kinase JAK1
  • JAK1B
  • Janus kinase 1EC 2.7.10.2
  • JTK3

Background

JAK1 (Janus kinase 1) a 130 kD non-receptor protein tyrosine kinase family and a member of the JAK subfamily is a widely expressed, membrane associated kinase involved in signal transduction pathways from interferon alpha, beta, and gamma receptors, all class II receptors, cytokine receptors that utilize the common gamma chain subunit for signaling, and cytokine receptors that utilize the gp130 subunit for signaling. JAK1 phosphorylates Stat proteins and SHP2. This kinase is constitutively expressed, but expression can be increased with interferon- alpha and interferon- gamma stimulation through transcription. JAK1 activity is increased by cytokine receptor-ligand binding and can be decreased by SOCS proteins, SHP2 protein phosphatase, and PPAR agonists. JAK1 is modified by phosphorylation and has been shown to associate with IFN- alpha receptor beta chain, IFN- gamma receptor alpha chain, IL-10 receptor alpha chain, IL-2 receptor beta chain, and IL-4 receptor alpha chain. The Poly6044 antibody recognizes the N-terminal region of human JAK1 and has been shown to be useful for Western blotting.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Jak1 Antibody (NBP1-31815)

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Blogs on Jak1.

Using a STAT3 antibody in chromatin immunoprecipitation (ChIP)
Signal transducer and activator of transcription 3 (STAT3) is an important oncogenic transcriptional factor that mediates tumor induced immune suppression.  Specifically, STAT3 transmits signals from cytokines and growth factor receptors in the pla...  Read full blog post.

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Bioinformatics

Gene Symbol JAK1
Entrez