Jagged 1 Antibody (J1G53-3) - Azide and BSA Free

Western Blot: Jagged 1 Antibody (J1G53-3) [NBP3-11671] -

Immunohistochemistry: Jagged 1 Antibody (J1G53-3) [NBP3-11671] - Immunohistochemical staining of JAG1 using Jagged 1 antibody (J1G53-3) in Pancreas, Acini and Duct (2.5 ug/ml). This antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.

Flow Cytometry: Jagged 1 Antibody (J1G53-3) [NBP3-11671] - FACS staining of human Lymphocyte(CD4+) using Jagged 1 antibody (J1G53-3) (PerCP).

Surface Plasmon Resonance: Jagged 1 Antibody (J1G53-3) [NBP3-11671] - SPR analysis of the interaction between Jagged 1 antibody (J1G53-3) derivative hJagged1-Fc fusion protein. SPR response was taken when 13, 6.5, 3.25, 1.625, and 0.8125nM of J1G53-3 were injected onto the hJagged1-Fc fusion protein. J1G53-3 exhibited the highest binding affinity, and its property and binding ability was studied in detail by spectroscopy and surface Plasmon resonance (SPR). The result acquired through SPR was as follows: Kd value was 1.08nM and value of chi-square was 0.0237 through SPR analysis.

• Reactivity: Hu
• Applications: WB, Flow, SPR
• Clone: J1G53-3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Jagged 1 Antibody (J1G53-3) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Jagged 1 Antibody (J1G53-3) - Azide and BSA Free (NBP3-11671) is a monoclonal antibody validated for use in WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human Jagged 1.
• Specificity: Recognizes human Jagged 1. Detects a band of ~180kDa by Western blot.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: JAG1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Surface Plasmon Resonance
  • Western Blot
• Application Notes: Western blot: 1:2000-1:5000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um-filtered solution in PBS, pH 7.4
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Jagged 1 Antibody (J1G53-3) - Azide and BSA Free

• AGS
• AHDMGC104644
• Alagille syndrome
• AWS
• CD339 antigen
• CD339
• HJ1
• JAG1
• Jagged 1
• Jagged1
• JAGL1
• protein jagged-1

Background

The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is theligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations thatalter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play arole in hematopoiesis. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: JAG1