JAB1 Antibody (8H8.5) - Azide and BSA Free

• Reactivity: Hu, Mu, Dr
• Applications: WB, IP
• Clone: 8H8.5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

JAB1 Antibody (8H8.5) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse JAB1 Antibody (8H8.5) - Azide and BSA Free (NB200-312) is a monoclonal antibody validated for use in WB and IP. Anti-JAB1 Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The full-length mouse JAB-1 gene expressed in E. coli.
• Localization: Nuclear/Cytoplasmic
• Specificity: This is specific for JAB-1.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: COPS5
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for JAB1 Antibody (8H8.5) - Azide and BSA Free

• COP9 constitutive photomorphogenic homolog subunit 5 (Arabidopsis)
• COP9 signalosome complex subunit 5
• COPS5
• CSN5
• CSN5COP9 (constitutive photomorphogenic, Arabidopsis, homolog) subunit 5
• EC 3.4
• JAB1
• JAB138 kDa Mov34 homolog
• Jun activation domain-binding protein 1
• MOV-34
• SGN5
• SGN5MGC3149
• Signalosome subunit 5

Background

JAB-1 is a 38kDa protein which was originally described as a coactivator for cJUN. It is present in a large protein complex called the Signalosome, which has been proposed to play a signaling role in mammalian cells. This protein complex appears to be analogous to the COP9 complex in plants, which is important in light-mediated plant development. Although the specific function of JAB-1 in this complex is unclear, it has recently been implicated in targeting proteins for degradation by the proteasome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for JAB1 Antibody (NB200-312)(4)

Publications using NB200-312

• Mitsuhashi T, Yonemoto J, Sone H et al. In utero exposure to dioxin causes neocortical dysgenesis through the actions of p27kip1. Proc Natl Acad Sci USA 2010-09-01 [PMID: 20805476]
• da Silva Correia J, Miranda Y, Leonard N et al. The subunit CSN6 of the COP9 signalosome is cleaved during apoptosis. J Biol Chem 2007-04-01 [PMID: 17337451]
• Burger-Kentischer A, Finkelmeier D, Thiele M et al. Binding of JAB1/CSN5 to MIF is mediated by the MPN domain but is independent of the JAMM motif. FEBS Lett 2005-03-01 [PMID: 15757663]
• Doronkin S, Djagaeva I, Beckendorf SK. The COP9 signalosome promotes degradation of Cyclin E during early Drosophila oogenesis. Dev Cell 2003-05-01 [PMID: 12737805]

Bioinformatics

• Gene Symbol: COPS5