JAB1 Antibody (2A10)

Images

 
Western Blot: JAB1 Antibody (2A10) [NB120-495] - Sample (50 ug of whole cell lysate) A: Rat liver 10% SDS PAGE diluted at 1:1000
Immunohistochemistry: JAB1 Antibody (2A10) [NB120-495]
Western Blot: JAB1 Antibody (2A10) [NB120-495] - Jab1 antibody [2A10.8] Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE JAB1 Antibody diluted at 1:10000
Western Blot: JAB1 Antibody (2A10) [NB120-495] - Jab1 antibody detects Jab1 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Jab1 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, DrSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clone
2A10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

JAB1 Antibody (2A10) Summary

Immunogen
The full-length mouse JAB-1 gene expressed in E. coli.
Localization
Cytoplasmic and Nuclear
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
COPS5
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunoprecipitation 0.5-1 ug/ml
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB120-495 in the following applications:

  • 7 publications
  • WB
    7 publications

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for JAB1 Antibody (2A10)

  • COP9 constitutive photomorphogenic homolog subunit 5 (Arabidopsis)
  • COP9 signalosome complex subunit 5
  • COPS5
  • CSN5
  • CSN5COP9 (constitutive photomorphogenic, Arabidopsis, homolog) subunit 5
  • EC 3.4
  • JAB1
  • JAB138 kDa Mov34 homolog
  • Jun activation domain-binding protein 1
  • MOV-34
  • SGN5
  • SGN5MGC3149
  • Signalosome subunit 5

Background

Jun activation domain-binding protein 1 (JAB1) also designated COP9 subunit 5 (COPS5) or SGN5 is a coactivator of AP1 transcription factor that also promotes degradation of the cyclin-dependent kinase inhibitor p27Kip1. JAB1 interacts with c-Jun AP1 containing complexes, and enhances transactivation from AP1 dependent promoters. It also interacts with Jun D but not with Jun B or v-Jun. JAB1 is highly conserved in evolution and is widely expressed in mammalian tissues. It is localized both the nucleus and the cytoplasm. It interacts with the cytoplasmic domain of the alphaL/beta2 integrin LFA1. Following LFA1 engagement the nuclear pool of JAB1 increases and activation of an AP1 driven promoter is enhanced. Interaction of JAB1 with the nuclear progesterone receptor and the steroid receptor activator (SRC1) was reported. JAB1 is a stability and activity regulator of Hypoxia inducible factor 1 (HIF1), a transcription factor that controls activation of several genes responsive to the cellular oxygen tension. The macrophage migration inhibitory factor (MIF) associates with JAB1 in the cytosol near the plasma membrane. Endogenous MIF inhibits JAB1 induced AP1 transcriptional activity. JAB1 is a subunit of the COP9 regulatory complex. COP9 cleaves the ubiquitin-like protein Nedd8 from the Cul1 subunit of SCF ubiquitin ligases. A metalloprotease motif in JAB1 plays a role in this isopeptidase activity. Breakdown of the cyclin dependent kinase inhibitor p27Kip1 is promoted by JAB1. The latter expression in several cancers inversely correlates with p27Kip1 and may reflect tumor aggressiveness. A possible involvement of JAB1 in atherosclerosis was also reported. Involvement of JAB1 in degradation of the suppressors p53 and smad4 was described recently.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for JAB1 Antibody (NB120-495)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(7)
WB
(7)
All Applications
Filter By Species
Human
(7)
Mouse
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using NB120-495 Applications Species
Wang R, Wang H, Carrera I et al. COPS5 Overexpression Increases Amyloid Plaque Burden, Decreases Spinophilin-Immunoreactive-Puncta and Exacerbates Learning and Memory Deficits in the Mouse Brain J. Biol. Chem. 2015 Feb 20 [PMID: 25713139]
Wang H, Dey D, Carrera I et al. COPS5 (Jab1) increases beta-site processing of amyloid precursor protein and Abeta generation by stabilizing RanBP9 protein levels. J Biol Chem. 2013 Sep 13 [PMID: 23926111] (WB, ICC/IF, Mouse, Human) WB, ICC/IF Mouse, Human
Merk M, Zierow S, Leng L et al. The D-dopachrome tautomerase (DDT) gene product is a cytokine and functional homolog of macrophage migration inhibitory factor (MIF). Proc Natl Acad Sci USA 2011 Aug [PMID: 21817065] (ICC/IF, WB, Human) ICC/IF, WB Human
McCall CM, Miliani de Marval PL, Chastain PD 2nd et al. Human immunodeficiency virus type 1 Vpr-binding protein VprBP, a WD40 protein associated with the DDB1-CUL4 E3 ubiquitin ligase, is essential for DNA replication and embryonic development. Mol Cell Biol 2008 Sep [PMID: 18606781] (ICC/IF, WB, Human) ICC/IF, WB Human
Yun J, Tomida A, Andoh T et al. Interaction between glucose-regulated destruction domain of DNA topoisomerase IIalpha and MPN domain of Jab1/CSN5. J Biol Chem 2004 Jul [PMID: 15126503] (ICC/IF, WB, Human) ICC/IF, WB Human
Doronkin S, Djagaeva I, Beckendorf SK. CSN5/Jab1 mutations affect axis formation in the Drosophila oocyte by activating a meiotic checkpoint. Development 2002 Nov [PMID: 12397113] (ICC/IF, WB, Human) ICC/IF, WB Human
Korbonits M, Chahal HS, Kaltsas G et al. Expression of phosphorylated p27(kip1) protein and Jun activation domain-binding protein 1 in human pituitary tumors. J Clin Endocrinol Metab 2002 Jun [PMID: 12050228] (ICC/IF, WB, Human) ICC/IF, WB Human
Gemmill RM, Bemis LT, Lee JP et al. The TRC8 hereditary kidney cancer gene suppresses growth and functions with VHL in a common pathway. Oncogene 2002 May [PMID: 12032852] (ICC/IF, WB, Human) ICC/IF, WB Human

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB120-495AF405
Alexa Fluor 488 Labeled NB120-495AF488
Alexa Fluor 647 Labeled NB120-495AF647
Alexa Fluor 700 Labeled NB120-495AF700
DyLight 350 Labeled NB120-495UV
DyLight 405 Labeled NB120-495V
DyLight 488 Labeled NB120-495G
DyLight 680 Labeled NB120-495FR
DyLight 755 Labeled NB120-495IR

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Bioinformatics

Gene Symbol COPS5
Entrez
Uniprot