ITIH4 Antibody

Western Blot: ITIH4 Antibody [NBP1-78286] (0.03ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: ITIH4 Antibody [NBP1-78286] - Staining of paraffin embedded Human Testis at dilution of 5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ITIH4 Antibody Summary

• Immunogen: Peptide with sequence C-SDDGRRTLRVQGND corresponding to C-Terminus according to NP_002209.2, NP_001159921.1.
• Specificity: This antibody is expected to recognize both reported isoforms (NP_002209.2; NP_001159921.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ITIH4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.03 - 0.1 ug/ml
• Application Notes: WB: Approx. 150 kDa band observed in human placenta lysates (calculated MW of 103 kDa band according to NP_002209.2). The observed molecular weight corresponds to the glycosylated form.
• Theoretical MW: 103 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ITIH4 Antibody

• DKFZp686G21125
• gp120
• H4P
• heavy chain-like, 1
• IHRP
• inter-alpha (globulin) inhibitor H4 (plasma Kallikrein-sensitive glycoprotein)
• inter-alpha-trypsin inhibitor family heavy chain-related protein
• inter-alpha-trypsin inhibitor heavy chain H4
• ITIH4
• ITI-HC4
• ITIHL1
• PK120
• PK-120
• plasma kallikrein-sensitive glycoprotein 120
• PRO1851

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ITIH4 Antibody (NBP1-78286)(1)

Publication using NBP1-78286

• Tang Y, Kitisin K, Jogunoori W et al. Progenitor/stem cells give rise to liver cancer due to aberrant TGF-beta and IL-6 signaling. Proc Natl Acad Sci U S A 2008-02-01 [PMID: 18263735]

Bioinformatics

• Gene Symbol: ITIH4
• Entrez:
  • 3700(Human)
• Uniprot:
  • Q14624()