ITIH4 Antibody

Western blot shows human plasma. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ITIH4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8157) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ITIH4 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

ITIH4 Antibody Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human ITIH4
  Glu29-Leu930 (Ile85Asn, Pro698Thr)
  Accession # Q14624
• Specificity: Detects human ITIH4 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human ITIH1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: ITIH4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ITIH4 Antibody

• DKFZp686G21125
• gp120
• H4P
• heavy chain-like, 1
• IHRP
• inter-alpha (globulin) inhibitor H4 (plasma Kallikrein-sensitive glycoprotein)
• inter-alpha-trypsin inhibitor family heavy chain-related protein
• inter-alpha-trypsin inhibitor heavy chain H4
• ITIH4
• ITI-HC4
• ITIHL1
• PK120
• PK-120
• plasma kallikrein-sensitive glycoprotein 120
• PRO1851

Background

The inter-alpha -trypsin inhibitor heavy chain (ITIH4), also known as IHRP (inter-alpha -trypsin heavy chain-related protein), PK-120 (plasma kallikrein-sensitive glycoprotein 120), or Gp120 (glycoprotein 120), is a secreted, 120 kDa, 930 amino acid (aa), N-glycosylated Type II acute phase protein. It is upregulated in response to trauma such as ischemic stroke or myocardial infarction, and functions as a trypsin inhibitor. It is activated by Kallikrein by cleavage between aa 688-689, creating 100 kDa and 35 kDa portions. Mature human ITIH4 shares 66% aa sequence identity with mouse and rat ITIH4. Human isoforms lacking aa 621-650 (914, 900 and 845 aa forms), aa 727-765 and 851-866 (845 aa) and having 14 aa inserted after aa 727 (914 aa) have been described. Proteolytic cleavage by plasma kallikrein produces a 100 kDa fragment that is further processed to 70 kDa, and 35 kDa fragments. An O-glycosylated form is reported in urine, but not in plasma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ITIH4 Antibody (AF8157)(2)

Publications using AF8157

• Lee, H;Assaraf, R;Subramanian, S;Goetschius, D;Bieri, J;DiNunno, NM;Leisi, R;Bator, CM;Hafenstein, SL;Ros, C; Infectious parvovirus B19 circulates in the blood coated with active host protease inhibitors Nature communications 2024-11-05 [PMID: 39500886] (Immunoprecipitation, Virus)
• Z Jia, L Liu, S Zhang, X Zhao, L Luo, Y Tang, B Shen, M Chen Proteomics changes after negative pressure wound therapy in diabetic foot ulcers Molecular Medicine Reports, 2021-10-05;24(6):. 2021-10-05 [PMID: 34608502] (Western Blot, Human)

Bioinformatics

• Gene Symbol: ITIH4
• Uniprot:
  • Q14624()