ITIH2 Antibody (1023637) [Unconjugated]

Recombinant Human ITIH2 protein was serially diluted 2-fold and captured by Mouse Anti-Human ITIH2 Monoclonal Antibody (Catalog # MAB101993) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human ITIH2 Monoclonal Antibody (MAB101991) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

• Reactivity: Hu
• Applications: ELISA
• Clone: 1023637
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

ITIH2 Antibody (1023637) [Unconjugated] Summary

• Immunogen: Spodoptera frugiperda Sf 9 (baculovirus)-derived human ITIH2
  Ser55-Asp702
  Accession # P19823
• Specificity: Detects human ITH2 in ELISA.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ITIH2 Antibody (1023637) [Unconjugated]

• H2P
• IGHEP2
• Inter-Alpha (Globulin) Inhibitor H2
• inter-alpha (globulin) inhibitor, H2 polypeptide
• Inter-alpha-inhibitor heavy chain 2
• Inter-alpha-trypsin inhibitor complex component II
• inter-alpha-trypsin inhibitor heavy chain H2
• Intin2
• ITI Heavy Chain H2
• ITIH2
• ITI-H2
• ITI-HC2
• Serum-derived hyaluronan-associated protein
• SHAP

Background

The ITIs are a family of plasma protease inhibitors contributing to extracellular matrix stabilization by covalent linkage to hyaluronan.  ITIH2 is a binding protein between hyaluronan and other matrix proteins.  It regulates the localization, synthesis and degradation of hyaluronan.  ITIH molecules are shown to play a role in carcinogenesis and inflammation.  ITIH genes are downregulated in multiple human solid tumors.  This downregulation contributes to initiation and/or progression of malignancy. 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P19823()