ITCH/AIP4 Antibody - BSA Free

Western Blot: ITCH/AIP4 Antibody [NBP2-81837] - Western blot analysis of ITCH/AIP4 in 3T3 cell lysate with ITCH/AIP4 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

ITCH/AIP4 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ITCH/AIP4 Antibody - BSA Free (NBP2-81837) is a polyclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: ITCH antibody was raised against a 15 amino acid peptide near the amino terminus of human ITCH . The immunogen is located within amino acids 40 - 90 of ITCH. Amino Acid Squence: NNTNSPKWKQPLTV
• Specificity: ITCH/AIP4 antibody is human, mouse and rat reactive. At least three isoforms of ITCH/AIP4 are known to exist; this antibody only recognizes the two longest isoforms. This antibody is predicted to not cross-react with other members of the Nedd4 protein family.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ITCH
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1-2 ug/mL
• Theoretical MW: 99 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for ITCH/AIP4 Antibody - BSA Free

• AIF4
• AIP4
• AIP4dJ468O1.1 (atrophin 1 interacting protein 4 (AIP4))
• atrophin-1 interacting protein 4
• Atrophin-1-interacting protein 4
• dJ468O1.1
• EC 6.3.2
• EC 6.3.2.-
• ITCH
• itchy (mouse homolog) E3 ubiquitin protein ligase
• itchy E3 ubiquitin protein ligase homolog (mouse)
• itchy homolog E3 ubiquitin protein ligase
• NAPP1
• NAPP1E3 ubiquitin-protein ligase Itchy homolog
• NFE2-associated polypeptide 1
• ubiquitin protein ligase ITCH

Background

Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral and pallidoluysian atrophy. The protein encoded by this gene interacts with atrophin-1. This encoded protein is a closely related member of the NEDD4-like protein family. This family of proteins are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. This encoded protein contains four tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. It can act as a transcriptional corepressor of p45/NFE2 and may participate in the regulation of immune responses by modifying Notch-mediated signaling. It is highly similar to the mouse Itch protein, which has been implicated in the regulation and differentiation of erythroid and lymphoid cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITCH