Isopeptidase T/USP5 Recombinant Protein Antigen

• Reactivity: Hu
• Applications: AC

Isopeptidase T/USP5 Recombinant Protein Antigen Summary

• Description: A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human USP5.
  
  

  Source: E. coli

  Amino Acid Sequence: YVDKLEKIFQNAPTDPTQDFSTQVAKLGHGLLSGEYSKPVPESGDGERVPEQKEVQDGIAPRMFKALIGKGHPEFSTNRQQDAQEFFLHLINMVERNCRSSENPNEVF

  Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

  This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein Antigen
• Gene: USP5
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Antibody Competition 10 - 100 molar excess
• Application Notes: This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP1-83089.

  It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

  For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

• Theoretical MW: 30 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1M Urea, pH 7.4.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for Isopeptidase T/USP5 Recombinant Protein Antigen

• Deubiquitinating enzyme 5
• EC 3.1.2.15
• EC 3.4.19.12
• Isopeptidase T
• IsoT
• ISOTubiquitin carboxyl-terminal hydrolase 5
• ubiquitin isopeptidase T
• ubiquitin specific peptidase 5 (isopeptidase T)
• ubiquitin specific protease 5 (isopeptidase T)
• ubiquitin thioesterase 5
• Ubiquitin thiolesterase 5
• ubiquitin-specific protease-5 (ubiquitin isopeptidase T)
• Ubiquitin-specific-processing protease 5
• USP5

Background

Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific processing protease 5 (USP5) is a member of this family. The upregulation of USP5 has been found to be associated with fetal Down syndrome. Alternate names for USP include ubiquitin carboxyl-terminal hydrolase 5, ubiquitin thioesterase 5, ubiquitin-specific-processing protease 5, deubuiquitinating enzyme 5, isopeptidase T, and ISOT.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: USP5