IRP2 Antibody


Western Blot: IRP2 Antibody [NB100-1797] - Detection of IRP2 in mouse liver lysate.
Immunocytochemistry/ Immunofluorescence: IRP2 Antibody [NB100-1797] - IRP2 antibody was tested in NIH-3T3 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
1 mg/ml

IRP2 Antibody Summary

A synthetic peptide made to an N-terminal portion of the human IRP2 protein sequence (between residues 1-100). [UniProt P48200]
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-Glycine and 0.15M NaCl
0.05% Sodium Azide
1 mg/ml
Immunogen affinity purified


  • Western Blot 1:500
  • Immunocytochemistry/Immunofluorescence 1:500
Application Notes
This IRP2 antibody is useful for Western blot and Immunocytochemistry/Immunofluorescence. In ICC/IF, cytoplasmic staining was observed in NIH-3T3 cells.
Read Publications using
NB100-1797 in the following applications:

  • WB
    1 publication

Reactivity Notes

Mouse.. Immunogen has 72% homology to rat. Human reactivity reported in scientific literature (PMID: 21865595)

Alternate Names for IRP2 Antibody

  • ACO3
  • EC
  • FLJ23381
  • IRE-BP 2
  • Iron regulatory protein 2
  • iron-responsive element binding protein 2
  • iron-responsive element-binding protein 2


Iron regulatory proteins -1 and -2 (IRP1, IRP2) are homologous cytoplasmic RNA-binding proteins that controls cellular iron metabolism and maintain systemic iron homeostasis in vertebrates along with serving some additional iron-independent functions. IRP2 belongs to aconitase/IPM isomerase family and it binds to IRES (iron-responsive elements), which are stem-loop structures found in 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. IRP2-IRE binding results in the repression of its mRNA translation and its binding to transferrin receptor mRNA inhibits the degradation of this otherwise quickly degradable mRNA. IRP2 is a substrate of FBXL5, an F-box protein that assembles together with Skp1, Cul1 and Rbx1 into an E3 ubiquitin ligase complex. When iron/O2 are high, IRP2 gets ubiquitinated/degraded by SCF complex containing FBXL5, whereas under conditions of low iron/O2, FBXL5 is degraded preventing IRP2 ubiquitination leading to its RNA-binding activity. IRP2 deficient mice depicts microcytosis, misregulated iron metabolism (in CNS, intestine as well as liver associated with FTH1 and FTL up-regulation) and iron deficiency in splenic macrophages associated with down-regulation of FTH1, FTL, and FPN1.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ELISA, PA
Species: Hu, Bv
Applications: WB, ELISA, IHC, IHC-P, S-ELISA
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu
Applications: WB, ELISA, S-ELISA
Species: Hu
Applications: WB, IHC
Species: Hu, Mu, Rt, Ch
Applications: WB, ELISA, IHC, IHC-P
Species: Hu, Mu, Rt, Bv, Ca
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ELISA, ICC/IF, S-ELISA
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu
Applications: WB, IHC, IHC-P

Publications for IRP2 Antibody (NB100-1797)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.

Filter By Application
All Applications
Filter By Species
All Species

Reviews for IRP2 Antibody (NB100-1797) (0)

There are no reviews for IRP2 Antibody (NB100-1797). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

View specific protocols for IRP2 Antibody (NB100-1797): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IRP2 Antibody (NB100-1797) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-1797AF405
Alexa Fluor 488 Labeled NB100-1797AF488
Alexa Fluor 647 Labeled NB100-1797AF647
Alexa Fluor 700 Labeled NB100-1797AF700
Biotin Labeled NB100-1797B
DyLight 405 Labeled NB100-1797V
DyLight 488 Labeled NB100-1797G
DyLight 550 Labeled NB100-1797R
DyLight 650 Labeled NB100-1797C
FITC Labeled NB100-1797F
HRP Labeled NB100-1797H

Additional IRP2 Antibody Products

Related Products by Gene

Bioinformatics Tool for IRP2 Antibody (NB100-1797)

Discover related pathways, diseases and genes to IRP2 Antibody (NB100-1797). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IRP2 Antibody (NB100-1797)

Discover more about diseases related to IRP2 Antibody (NB100-1797).

Pathways for IRP2 Antibody (NB100-1797)

View related products by pathway.

Research Areas for IRP2 Antibody (NB100-1797)

Find related products by research area.

Blogs on IRP2

There are no specific blogs for IRP2, but you can read our latest blog posts.

Contact Information

Product PDFs


Gene Symbol IREB2

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NB100-1797 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought