Iroquois-class homeodomain protein IRX-5 Antibody (8A12) - Azide and BSA Free

Western Blot: Iroquois-class homeodomain protein IRX-5 Antibody (8A12) [H00010265-M06] - Analysis of IRX5 expression in Raw 264.7.

Immunocytochemistry/Immunofluorescence: Iroquois-class homeodomain protein IRX-5 Antibody (8A12) [H00010265-M06] - Analysis of monoclonal antibody to IRX5 on HeLa cell. Antibody concentration 10 ug/ml

Western Blot: Iroquois-class homeodomain protein IRX-5 Antibody (8A12) [H00010265-M06] - Analysis of IRX5 expression in NIH/3T3 (Cat # L018V1).

ELISA: Iroquois-class homeodomain protein IRX-5 Antibody (8A12) [H00010265-M06] - Detection limit for recombinant GST tagged IRX5 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF
• Clone: 8A12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Iroquois-class homeodomain protein IRX-5 Antibody (8A12) - Azide and BSA Free Summary

• Immunogen: IRX5 (NP_005844, 204 a.a. ~ 264 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PQKPEDKADPEGPEAGGAEQKAASGCERLQGPPTPAGKETEGSLSDSDFKEPPSEGRLDAL
• Specificity: IRX5 - iroquois homeobox protein 5
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: IRX5
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Iroquois-class homeodomain protein IRX-5 Antibody (8A12) - Azide and BSA Free

• Homeodomain protein IRX-2A
• Homeodomain protein IRXB2
• iroquois homeobox 5
• Iroquois homeobox protein 5
• iroquois-class homeodomain protein IRX-5
• IRX2A
• IRX-2a
• IRXB2

Background

IRX5 is a member of the Iroquois homeobox gene family. Members of this family appear to play multiple roles during pattern formation of vertebrate embryos.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Iroquois-class homeodomain protein IRX-5 Antibody (H00010265-M06)(1)

Publication using H00010265-M06

• Ota H, Sakurai M, Gupta R et al. ADAR1 Forms a Complex with Dicer to Promote MicroRNA Processing and RNA-Induced Gene Silencing. Cell 2013-04-25 [PMID: 23622242] (WB, Mouse)

Bioinformatics

• Gene Symbol: IRX5
• Entrez:
  • 10265(Human)
• OMIM:
  • 606195(Human)
• Uniprot:
  • NP_005844(Human)