IRF8 Antibody (809926) [Allophycocyanin]

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 809926
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

IRF8 Antibody (809926) [Allophycocyanin] Summary

• Immunogen: E. coli-derived recombinant human IRF8
  Ala255-Val426
  Accession # Q02556
• Specificity: Detects human IRF8 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human IRF4 is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: IRF8
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Alternate Names for IRF8 Antibody (809926) [Allophycocyanin]

• ICSBP1
• ICSBPH-ICSBP
• interferon consensus sequence binding protein 1
• Interferon consensus sequence-binding protein
• interferon regulatory factor 8
• IRF8
• IRF-8ICSBP1

Background

Interferon regulatory factor 8 (IRF8) / Interferon consensus sequence binding protein (ICSBP) is a transcription factor of the interferon (IFN) regulatory factor (IRF) family. Proteins of this family are composed of a conserved DNA binding domain in the N terminal region and a divergent C terminal region that serves as the regulatory domain. The IRF family proteins bind to the IFN stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN alpha and IFN beta. IRF family proteins also control expression of IFN alpha and IFN beta regulated genes that are induced by viral infection. IRF8 specifically binds to the upstream regulatory region of type I IFN and IFN inducible MHC class I genes (the interferon consensus sequence (ICS)). Unlike IRF3, IRF8 appears to act as a negative regulator of IFN induced genes in most cases, but IRF8 mediates activation of NF kappaB by the toll like receptor 9 (TLR9) after stimulation by unmethylated CpG DNA in dendritic cells. Finally, it has been shown that IRF8 decreases bcl-2 expression and thus may play a role in chronic myelogenous leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IRF8