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IRF7 Antibody (3D9) [Alexa Fluor® 750]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
3D9
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 750

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-04309AF350
Alexa Fluor 405
NBP1-04309AF405
Alexa Fluor 488
NBP1-04309AF488
Alexa Fluor 532
NBP1-04309AF532
Alexa Fluor 594
NBP1-04309AF594
Alexa Fluor 647
NBP1-04309AF647
Alexa Fluor 700
NBP1-04309AF700
Biotin
NBP1-04309B
DyLight 350
NBP1-04309UV
DyLight 405
NBP1-04309V
DyLight 488
NBP1-04309G
DyLight 550
NBP1-04309R
DyLight 594
NBP1-04309DL594
DyLight 650
NBP1-04309C
DyLight 680
NBP1-04309FR
DyLight 755
NBP1-04309IR
FITC
NBP1-04309F
HRP
NBP1-04309H
Unconjugated
NBP1-04309
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IRF7 Antibody (3D9) [Alexa Fluor® 750] Summary

Immunogen
Recombinant human IRF7 (1-150aa) purified from E. coli (NP_001563).
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
IRF7
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for IRF7 Antibody (3D9) [Alexa Fluor® 750]

  • interferon regulatory factor 7
  • interferon regulatory factor-7H
  • IRF-7
  • IRF7A
  • IRF-7H

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: IFN-alpha 1 Antibody [NBP2-75930] - staining IFNA1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunocytochemistry/Immunofluorescence: IRF3 Antibody (SD2062) [NBP2-67741] - Staining IRF3 in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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MuRF1/TRIM63
Antibody [Unconjugate
AF5366
Western blot shows lysates of human heart, mouse heart, and rat muscle tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat MuRF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5366) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MuRF1 at approximately 41‑44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Simple Western lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for MuRF1/TRIM63 at approximately 46 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat MuRF1/TRIM63 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5366) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
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(26C593.2)
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Simple Western: TLR9 Antibody (26C593.2) [NBP2-24729] - Lane view shows a specific band for TLR9 in 0.5 mg/ml of Ramos lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system. Image using the Azide Free format of this antibody.
Flow Cytometry: TLR9 Antibody (26C593.2) [NBP2-24729] - Expression of TLR9 protein on epithelial cells. HNEC, Detroit-562 and FaDu were stained intracellularly with PE-Ab against TLR9 (open histograms) or appropriate isotype control (shaded histograms) and analyzed by flow cytometry. Representative pictures from one out of three independent experiments are shown. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0098239), licensed under a CC-BY licence.
Knockdown Validated: TLR9 Antibody (26C593.2) [NBP2-24729] - Expression of TLR-9 protein in HB cells before and after 48 hr of transfection. Beta-actin was used as an internal control. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092748) licensed under a CC-BY licence.
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1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.
(32 Reviews)
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TLR7 Antibody
NBP2-24906
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Flow Cytometry: TLR7 Antibody [NBP2-24906] - An intracellular stain was performed on THP-1 cells with NBP2-24906 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550.
Immunocytochemistry/Immunofluorescence: TLR7 Antibody [NBP2-24906] - Immunolocalization of TLR7 (A) and glial fibrillary acidic protein (GFAP) (B) in murine myenteric plexus. Most of the GFAP positive cells were also positive for TLR7 in the merged image (C, yellow staining). (Courtesy of Barajon I, et al., Journal of Histochemistry and Cytochemistry, 57(11): 1013-;1023, 2009.)
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IRF1 Antibody
AF4715
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NFkB p105/p50
Antibody (2J10D7)
NB100-56583
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Immunohistochemistry-Paraffin: RIG-I Antibody [NBP2-27419] - Formalin-fixed, paraffin-embedded human uterus stained with RIG-I antibody at 10 ug/ml.
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(40C1285.6)
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IRF5 Antibody
NB100-1092
Western Blot: IRF5 Antibody [NB100-1092] - Western blot analysis of Human Spleen (A) and A549 cell lysate (B) + peptide (C), 35 ug protein in RIPA buffer. Antibody at 1 ug/mL. Detected by chemiluminescence
Western Blot: IRF5 Antibody [NB100-1092] - Endogenous IRF5 knockdown analysis. Ramos B cells were nucleofected with scrambled (Scr) or IRF5 (KD) siRNAs and lysates from same nucleofection run on multiple independent blots for comparative analysis of seven different commercially available alpha-IRF5 antibodies. Arrows indicate detection of an appropriately sized band(s) corresponding to IRF5. Data are representative of three independent experiments. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep31002), licensed under a CC-BY licence.
Western Blot: IRF5 Antibody [NB100-1092] - Comparative analysis of IRF5 protein expression between immortalized lymphoid cell lines by immunoblot. Endogenous IRF5 protein expression in lysates (T, THP-1; R, Ramos B cells; J, Jurkat T cells) was detecting by running multiple independent blots for the analysis of antibody specificity; seven different commercially available alpha-IRF5 antibodies were evaluated. IRF5 expression was detected with the previously validated Cell Signaling antibody #3257 that is no longer available. beta-actin levels and apparent molecular weight standards are shown as loading controls and for size comparison, respectively. Arrows indicate detection of an appropriately sized band(s) corresponding to IRF5. Data are representative of three independent experiments. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep31002), licensed under a CC-BY licence.
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MyD88 Inhibitor
Peptide Set
NBP2-29328
MyD88 Inhibitor Peptide Set
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(86 Publications)
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Applications: ELISA
TBK1 Antibody
(108A429)
NB100-56705
Western Blot: TBK1 Antibody (108A429) [NB100-56705] - Analysis using Azide and BSA Free version of NB100-56705. Lysate from 293 cells untransfected (lane 1) and transfected with human TBK1 cDNA (lane 2) probed with TBK1 antibody at 2 ug/ml.
Immunocytochemistry/Immunofluorescence: TBK1 Antibody (108A429) [NB100-56705] - Antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). A dilution of 1:10 was used. Image objective 40x.
Immunohistochemistry-Paraffin: TBK1 Antibody (108A429) [NB100-56705] - Immunohistochemistry: TBK1 Antibody (108A429) - Azide and BSA Free [NBP2-33243] - Staining on human testis (Formalin-Fixed Paraffin-Embedded (FFPE)) Image from the Azide and BSA Free version of this antibody.
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IL-6 [HRP]
D6050
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TRIF/TICAM1
Antibody
NB120-13810
Western Blot: TRIF/TICAM1 Antibody [NB120-13810] - Analysis of TRIF in mouse spleen probed with TRIF antibody, in A: presence and B: absence of immunizing peptide, at 1:1000.
Immunocytochemistry/Immunofluorescence: TRIF/TICAM1 Antibody [NB120-13810] - TRIF antibody was tested in A431 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40X. An antibody dilution of 1:10 was used.
Western Blot: TRIF/TICAM1 Antibody [NB120-13810] - Analysis of TRIF in mouse A: Intestine. B: Spleen. C: RAW lysate using TRIF antibody at 1:1000 dilution.
(31 Publications)
Species: Hu, Mu, Rt, Po
Applications: WB, Simple Western, IB, ICC/IF, IP
IRF7 Antibody
(3D9) [Alexa Fluor ...
NBP1-04309AF750
IRF7 Antibody (3D9) [Alexa Fluor® 750]
Species: Hu
Applications: WB, ELISA

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-04309AF350
Alexa Fluor 405 NBP1-04309AF405
Alexa Fluor 488 NBP1-04309AF488
Alexa Fluor 532 NBP1-04309AF532
Alexa Fluor 594 NBP1-04309AF594
Alexa Fluor 647 NBP1-04309AF647
Alexa Fluor 700 NBP1-04309AF700
Alexa Fluor 750 NBP1-04309AF750
Biotin NBP1-04309B
DyLight 350 NBP1-04309UV
DyLight 405 NBP1-04309V
DyLight 488 NBP1-04309G
DyLight 550 NBP1-04309R
DyLight 594 NBP1-04309DL594
DyLight 650 NBP1-04309C
DyLight 680 NBP1-04309FR
DyLight 755 NBP1-04309IR
FITC NBP1-04309F
HRP NBP1-04309H
Learn about Custom Antibody Labeling

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Array NBP1-04309AF750

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Blogs on IRF7.

Check out the latest blog posts on IRF7.
TLR7 and Immune Response Regulation
Toll-like receptor 7 (TLR7) is a protein encoded by the TLR7 gene in humans and is a member of TLR family. TLRs controls host immune response against pathogens (e.g. viruses, bacteria and fungi) through recognition of pathogen-associated molecular pat...  Read full blog post.
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Bioinformatics

Gene Symbol IRF7