IRF5 Antibody (1H6) - Azide and BSA Free

Western Blot: IRF5 Antibody (1H6) [H00003663-M03] - Analysis of IRF5 expression in NIH/3T3 (Cat # L018V1).

Immunocytochemistry/Immunofluorescence: IRF5 Antibody (1H6) [H00003663-M03] - Analysis of monoclonal antibody to IRF5 on A-431 cell. Antibody concentration 10 ug/ml

Western Blot: IRF5 Antibody (1H6) [H00003663-M03] - Analysis of IRF5 expression in A-431 (Cat # L015V1).

ELISA: IRF5 Antibody (1H6) [H00003663-M03] - Detection limit for recombinant GST tagged IRF5 is approximately 0.1ng/ml as a capture antibody.

Immunoprecipitation: IRF5 Antibody (1H6) [H00003663-M03] - Analysis of IRF5 transfected lysate using anti-IRF5 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with IRF5 mouse polyclonal antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Flow, ICC/IF, IP
• Clone: 1H6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

IRF5 Antibody (1H6) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: IRF5 (NP_002191, 395 a.a. ~ 504 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TPPPFEIFFCFGEEWPDRKPREKKLITVQVVPVAARLLLEMFSGELSWSADSIRLQISNPDLKDRMVEQFKELHHIWQSQQRLQPVAQAPPGAGLGVGQGPWPMHPAGMQ
• Specificity: IRF5 - interferon regulatory factor 5
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: IRF5
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA. Use in FLOW reported in scientific literature (PMID: 30593537).

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for IRF5 Antibody (1H6) - Azide and BSA Free

• IBD14
• interferon regulatory factor 5
• IRF5
• IRF-5
• SLEB10

Background

This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Alternative splice variants encoding different isoforms exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IRF5 Antibody (H00003663-M03)(2)

Publications using H00003663-M03

• Hedl M, Yan J, Witt H, Abraham C. IRF5 Is Required for Bacterial Clearance in Human M1-Polarized Macrophages, and IRF5 Immune-Mediated Disease Risk Variants Modulate This Outcome J. Immunol. 2018-12-28 [PMID: 30593537] (FLOW, Human)
• Hedl M, Yan J, Abraham C. IRF5 and IRF5 Disease-Risk Variants Increase Glycolysis and Human M1 Macrophage Polarization by Regulating Proximal Signaling and Akt2 Activation. Cell Rep. 2016-08-30 [PMID: 27545875]

Bioinformatics

• Gene Symbol: IRF5
• Entrez:
  • 3663(Human)
• OMIM:
  • 607218(Human)
• Uniprot:
  • NP_002191()