IRF5 Antibody (10T1) - BSA Free

Western Blot: IRF5 Antibody (10T1) [NBP1-04307] - The extracts of Ramos, THP-1, A20 and NIH3T3 were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IRF5 antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunocytochemistry/Immunofluorescence: IRF5 Antibody (10T1) [NBP1-04307] - Analysis in THP-1 cells line, stained with DAPI (Blue) for nucleus staining and antibody at 1:100 with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Immunohistochemistry-Paraffin: IRF5 Antibody (10T1) [NBP1-04307] - Human IRF-5 antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).

Flow (Intracellular): IRF5 Antibody (10T1) [NBP1-04307] - An intracellular stain was performed on THP-1 cells with IRF5 Antibody (10T1) NBP1-04307PE (blue) and a matched isotype control(orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.

Flow Cytometry: IRF5 Antibody (10T1) [NBP1-04307] - Flow cytometry analysis of IRF5 in THP-1 cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

• Reactivity: Hu
• Applications: WB, ELISA, Flow, Flow-IC, ICC/IF, IHC, IHC-P
• Clone: 10T1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

IRF5 Antibody (10T1) - BSA Free Summary

• Immunogen: Recombinant human IRF-5 (176-240 aa) purified from E. coli
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: IRF5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow (Intracellular)
  • Flow Cytometry 2-5ug
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for IRF5 Antibody (10T1) - BSA Free

• IBD14
• interferon regulatory factor 5
• IRF5
• IRF-5
• SLEB10

Background

Interferon regulatory factor 5(IRF-5) is a member of IRF family, which plays a critical role in the regulation of genes induced by viral infection, immunostimulation and cell growth regulation. The expression of IRF-5 is limited to lymphoid organs, dendritic cells, and peripheral blood lymphocytes, but is not expressed in numerous leukemia and lymphoma cell lines. Three IRFs(IRF-3, IRF-5, IRF-7) function as direct transducers of virus-mediated signaling and play a critical role in the expression of type I interferon genes and some chemokines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for IRF5 Antibody (NBP1-04307) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Sarah Miller WB Human 08/30/2021

Summary

• Application: Western Blot
• Sample Tested: Dendritic cells
• Species: Human
• Lot: 10T1

Bioinformatics

• Gene Symbol: IRF5
• Uniprot:
  • NP_002191()
  • NP_116032.1(Human)
  • Q13568(Human)
  • Q13568(Human)
  • Q13568(Human)