IRF5 Antibody (10T1) - BSA Free

Images

 
Western Blot: IRF5 Antibody (10T1) [NBP1-04307] - The extracts of Ramos, THP-1, A20 and NIH3T3 were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IRF5 antibody (1:1,000). Proteins were ...read more
Immunocytochemistry/ Immunofluorescence: IRF5 Antibody (10T1) [NBP1-04307] - Analysis in THP-1 cells line, stained with DAPI (Blue) for nucleus staining and antibody at 1:100 with goat anti-mouse IgG-Alexa fluor 488 ...read more
Immunohistochemistry-Paraffin: IRF5 Antibody (10T1) [NBP1-04307] - Human IRF-5 antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using ...read more
Flow (Intracellular): IRF5 Antibody (10T1) [NBP1-04307] - An intracellular stain was performed on THP-1 cells with IRF5 Antibody (10T1) NBP1-04307PE (blue) and a matched isotype control(orange). Cells were fixed with ...read more
Flow Cytometry: IRF5 Antibody (10T1) [NBP1-04307] - Flow cytometry analysis of IRF5 in THP-1 cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clone
10T1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

IRF5 Antibody (10T1) - BSA Free Summary

Immunogen
Recombinant human IRF5 (176-240 aa) purified from E. coli
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
IRF5
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Flow (Intracellular)
  • Flow Cytometry 2-5ug
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:500-1:1000
Reviewed Applications
Read 1 Review rated 5
using
NBP1-04307 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for IRF5 Antibody (10T1) - BSA Free

  • IBD14
  • interferon regulatory factor 5
  • IRF5
  • IRF-5
  • SLEB10

Background

Interferon regulatory factor 5(IRF-5) is a member of IRF family, which plays a critical role in the regulation of genes induced by viral infection, immunostimulation and cell growth regulation. The expression of IRF-5 is limited to lymphoid organs, dendritic cells, and peripheral blood lymphocytes, but is not expressed in numerous leukemia and lymphoma cell lines. Three IRFs(IRF-3, IRF-5, IRF-7) function as direct transducers of virus-mediated signaling and play a critical role in the expression of type I interferon genes and some chemokines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IRF5 Antibody (NBP1-04307) (0)

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Review for IRF5 Antibody (NBP1-04307) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-04307:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot IRF5 NBP1-04307
Enlarge
5
reviewed by:
Verified Customer
WB Human 08/30/2021
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Summary

ApplicationWestern Blot
Sample TestedDendritic cells
SpeciesHuman
Lot10T1

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IRF5 Antibody (NBP1-04307) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Research Areas for IRF5 Antibody (NBP1-04307)

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Blogs on IRF5

There are no specific blogs for IRF5, but you can read our latest blog posts.
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Recent Reviews

5
5
1
4
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3
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2
0
1
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Verified Customer
08/30/2021
Application: WB
Species: Human

Bioinformatics

Gene Symbol IRF5
Uniprot