IRF4 Antibody (2D1) - Azide and BSA Free

Western Blot: IRF4 Antibody (2D1) [H00003662-M05] - Analysis of IRF4 expression in transfected 293T cell line by IRF4 monoclonal antibody (M05), clone 2D1. Lane 1: IRF4 transfected lysatE (51.8 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: IRF4 Antibody (2D1) [H00003662-M05] - Analysis of monoclonal antibody to IRF4 on HeLa cell. Antibody concentration 10 ug/ml

Western Blot: IRF4 Antibody (2D1) [H00003662-M05] - Analysis of IRF4 over-expressed 293 cell line, cotransfected with IRF4 Validated Chimera RNAi or non-transfected control. Blot probed with H00003662-M05. GAPDH (36.1 kDa) used as loading control.

Sandwich ELISA: IRF4 Antibody (2D1) [H00003662-M05] - Detection limit for recombinant GST tagged IRF4 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, KD
• Clone: 2D1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

IRF4 Antibody (2D1) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: IRF4 (NP_002451, 342 a.a. ~ 451 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LCNDRPNKLERDQTCKLFDTQQFLSELQAFAHHGRSLPRFQVTLCFGEEFPDPQRQRKLITAHVEPLLARQLYYFAQQNSGHFLRGYDLPEHISNPEDYHRSIRHSSIQE
• Specificity: IRF4 (2D1)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: IRF4
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Knockdown Validated
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for IRF4 Antibody (2D1) - Azide and BSA Free

• interferon regulatory factor 4
• IRF4
• IRF-4
• LSIRF
• LSIRFMUM1
• Lymphocyte-specific interferon regulatory factor
• Multiple myeloma oncogene 1
• MUM1
• NF-EM5

Background

Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IRF4
• Entrez:
  • 3662(Human)
• OMIM:
  • 601900(Human)
• Uniprot:
  • NP_002451(Human)