IRF1 Antibody (686703) [Alexa Fluor® 594]

• Reactivity: Hu
• Applications: WB
• Clone: 686703
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 594

IRF1 Antibody (686703) [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant human IRF1
  Thr147-Pro325
  Accession # P10914
• Specificity: Detects human IRF1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) IRF2 (aa 151-349), rhIRF3 (aa 206-427), rhIRF4 (aa 130-451), rhIRF5 (aa 214-395), rhIRF6 (aa 176-366), rhIRF7 (
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: IgM-specific Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IRF1 Antibody (686703) [Alexa Fluor® 594]

• interferon regulatory factor 1
• IRF1
• MAR
• MARIRF-1

Background

IRF1 (interferon regulatory factor 1) is a 45 kDa member of the IRF family of proteins. It is induced by TLR9 and INF-gamma stimulation, and positively regulates the expression of IFN-beta , iNOS and 1L-12 p35.  Human IRF1 is 325 amino acids (aa) in length. It contains an N-terminal DNA-binding basic region (aa 1-140) and an acidic C-terminus (aa 141-325). Lys275 and 299 can be SUMOylated, which blocks activity, or ubiquinated at Lys299, which initiates degradation. There is a splice variant that shows an 11 aa substitution for the C-terminal 86 amino acids. Over aa 171-325, human IRF1 shares 83% aa identity with both canine and porcine IRF1.   

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.