IP3R1 Antibody

Immunocytochemistry/Immunofluorescence: IP3R1 Antibody [NBP2-22458] - Immunolocalization of IP3 receptor type I in rat cerebellum.

• Reactivity: Mu, Rt, Ca
• Applications: WB, Simple Western, Flow, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.3 mg/ml

IP3R1 Antibody Summary

• Immunogen: Synthetic Peptide: N(1829) K K K D D E V D R D A P S R K K A K E(1848)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ITPR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 3 - 5 ug / 10^6 cells
  • Immunocytochemistry/ Immunofluorescence 2 ug/ml
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:250
  • Immunoprecipitation 1:10 - 1:500
  • Simple Western 1:25
  • Western Blot 1:1000
• Application Notes: WB: Detects an approx. 240 kDa protein representing IP3R-I from rat and mouse brain extract.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1.3 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for IP3R1 Antibody

• DKFZp313E1334
• DKFZp313N1434
• inositol 14,5-triphosphate receptor, type 1
• inositol 14,5-trisphosphate receptor type 1
• InsP3R1
• IP3 receptor isoform 1
• IP3 receptor
• IP3R 1
• IP3R
• IP3R1
• ITPR1
• SCA15
• SCA16
• spinocerebellar ataxia 15
• spinocerebellar ataxia 16
• Type 1 inositol 14,5-trisphosphate receptor
• Type 1 InsP3 receptor

Background

IP3R1 (inositol 1,4,5-triphosphate receptor type 1) is an intracellular calcium release channel that mediates calcium release from the endoplasmic reticulum. There are 3 mammalian InsP3R receptors: Insp3R1, Insp3R2, and Insp3R3. Defects in the gene which encodes InsP3R1, ITPR1, are the cause of spinocerebellar ataxia type 15 (SCA15).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IP3R1 Antibody (NBP2-22458)(2)

Publications using NBP2-22458

• Hema Saranya Ilamathi, Sara Benhammouda, Laurent Chatel-Chaix, Marc Germain Protocol for measuring interorganelle contact sites in primary cells using a modified proximity ligation assay. STAR protocols 2024-03-20 [PMID: 38393951]
• Ilamathi H, Benhammouda S, Lounas A et al. Contact sites between endoplasmic reticulum sheets and mitochondria regulate mitochondrial DNA replication and segregation iScience 2023-06-01 [PMID: 37534187] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: ITPR1
• Entrez:
  • 25262(Rat)