Involucrin Antibody (SPM259) [Allophycocyanin]

Images

 
Flow Cytometry: Involucrin Antibody (SPM259) [Allophycocyanin] [NBP2-34402APC] - Human keratinocytes. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Po, Ca, Pm, Mu(-)Species Glossary
Applications Flow
Clone
SPM259
Clonality
Monoclonal
Host
Mouse
Conjugate
Allophycocyanin

Order Details

Involucrin Antibody (SPM259) [Allophycocyanin] Summary

Immunogen
Purified involucrin from human keratinocytes
Localization
Cytoplasmic
Marker
Squamous Cell Terminal Differentiation Marker
Specificity
It recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120kDa protein. It stains the involucrin in a variety of sizes: 170kDa in MCF-7 cells, a doublet of ~115kDa and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal; expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
IVL
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
Reviewed Applications
Read 1 Review rated 5
using
NBP2-34402APC in the following application:

Reactivity Notes

Gorilla, Owl monkey

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Involucrin Antibody (SPM259) [Allophycocyanin]

  • involucrin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Involucrin Antibody (NBP2-34402APC) (0)

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Review for Involucrin Antibody (NBP2-34402APC) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-34402APC:
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Images Ratings Applications Species Date Details
Flow Cytometry Involucrin NBP2-34402APC
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5
reviewed by:
Julien COUTIER
Flow Human 08/23/2018
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Summary

ApplicationFlow Cytometry
Sample TestedHuman keratinocytes
SpeciesHuman
Lot3713-1XPABX180611-080318-APC

Comments

CommentsUsed at the dilution of 1/100e in washing buffer, after permeabilization with the cytofix/cytoperm kit (BD), 1H at 4°C.

Product General Protocols

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FAQs for Involucrin Antibody (NBP2-34402APC) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-34402AF350
Alexa Fluor 405 NBP2-34402AF405
Alexa Fluor 488 NBP2-34402AF488
Alexa Fluor 532 NBP2-34402AF532
Alexa Fluor 594 NBP2-34402AF594
Alexa Fluor 647 NBP2-34402AF647
Alexa Fluor 700 NBP2-34402AF700
Alexa Fluor 750 NBP2-34402AF750
Allophycocyanin NBP2-34402APC
Biotin NBP2-34402B
DyLight 350 NBP2-34402UV
DyLight 405 NBP2-34402V
DyLight 488 NBP2-34402G
DyLight 550 NBP2-34402R
DyLight 594 NBP2-34402DL594
DyLight 650 NBP2-34402C
DyLight 680 NBP2-34402FR
DyLight 755 NBP2-34402IR
FITC NBP2-34402F
HRP NBP2-34402H
Janelia Fluor 549 NBP2-34402JF549
Janelia Fluor 646 NBP2-34402JF646
PE NBP2-34402PE
PerCP NBP2-34402PCP

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Bioinformatics Tool for Involucrin Antibody (NBP2-34402APC)

Discover related pathways, diseases and genes to Involucrin Antibody (NBP2-34402APC). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Recent Reviews

5
5
1
4
0
3
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2
0
1
0

Julien COUTIER
08/23/2018
Application: Flow
Species: Human

Bioinformatics

Gene Symbol IVL