Involucrin Antibody (rIVRN/9323)

Immunohistochemistry-Paraffin: Involucrin Antibody (rIVRN/9323) [NBP3-23830] - Formalin-fixed, paraffin-embedded human skin stained with Involucrin Antibody (rIVRN/9323). Inset: PBS instead of primary antibody; secondary only negative control.

• Reactivity: Hu
• Applications: IHC
• Clone: rIVRN/9323
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Involucrin Antibody (rIVRN/9323) Summary

• Description: Store at 2 to 8C. Non-hazardous. No MSDS required.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant human full-length Involucrin protein (IVRN)
• Localization: Cytoplasm.
• Marker: Squamous Cell Terminal Differentiation Marker
• Specificity: It recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this monoclonal antibody reacts with a 120kDa protein. It stains the involucrin in a variety of sizes: 170kDa in MCF-7 cells, a doublet of ~115kDa and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig. Its epitope maps between codon 421-568 of human involucrin.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: IVL
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 0.1-0.2ug/ml
• Application Notes: Immunohistochemistry-Paraffin: 0.1-0.2ug/ml for 30 min at RT. Staining of formalin-fixed tissues is enhanced by digestion with trypsin or Protease XXV at 1mg/ml PBS for 5 min at 37C. Note than enzyme digestion is better than citrate buffer based epitope unmasking. Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A purified

Alternate Names for Involucrin Antibody (rIVRN/9323)

• involucrin

Background

Stains paraffin embedded human tissue, so will be useful for studies of changes in involucrin expression in pathological studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IVL