Intersectin 1 Antibody - BSA Free

Western Blot: Intersectin 1 Antibody [NBP3-41833] - Sample: Recombinant protein.

Immunohistochemistry-Paraffin: Intersectin 1 Antibody [NBP3-41833] - DAB staining on IHC-P; Samples: Rat Kidney Tissue; Primary Ab: 10ug/ml NBP3-41833; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

Western Blot: Intersectin 1 Antibody [NBP3-41833] - Sample: Mouse Gallbladder lysate; Primary Ab: 1ug/ml NBP3-41833; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

• Reactivity: Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

Intersectin 1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Intersectin 1 Antibody - BSA Free (NBP3-41833) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Immunized with recombinant Rat Intersectin 1 (Uniprot#: Q9WVE9; Met1-Gln227 (Uniprot#: Q9WVE9)
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ITSN1
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-20ug/mL
  • Immunohistochemistry-Paraffin 5-20ug/mL
  • Western Blot 0.01-2ug/mL

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH7.4, 50% glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Antigen and protein A Affinity-purified

Alternate Names for Intersectin 1 Antibody - BSA Free

• 11
• intersectin 1 (SH3 domain protein)
• intersectin short variant 12
• intersectin-1
• ITSN
• SH3 domain protein-1A
• SH3 domain-containing protein 1A
• Src homology 3 domain-containing protein

Background

Intersectin 1 is an adapter protein that may provide an indirect link between the endocytic membrane traffic and the actin assembly machinery. It may regulate the formation of clathrin-coated vesicles. Isoform 1 could be involved in brain-specific synaptic vesicle recycling. Intersectin 1 is overexpressed in brain from Down syndrome foetuses suggesting a gene dosage-dependent contribution to the abnormalities of Down syndrome. Intersectin 1 interacts with dynamin, CDC42, SNAP25 and SNAP23. It clusters several dynamin in a manner that is regulated by alternative splicing. Intersectin 1 also binds clathrin-associated proteins and other components of the endocytic machinery, such as SPIN90, EPS15, EPN1, EPN2 and STN2. Adapter protein that may provide indirect link between the endocytic membrane traffic and the actin assembly machinery. May regulate the formation of clathrin-coated vesicles. Isoform 1 could be involved in brain-specific synaptic vesicle recycling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITSN1