Integrin beta 1/CD29 Antibody (EP1041Y)

Product Discontinued

Integrin beta 1/CD29 Antibody (EP1041Y) Summary

• Immunogen: A synthetic peptide corresponding to the residue near the C-terminal of human Integrin beta-1 (CD29) was used as immunogen.
• Marker: Stem Cell Marker
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ITGB1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:70
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:250-500
  • Western Blot 1:1000-10000
• Application Notes: In Western blot this antibody detects a band at approximately 140-150 kDa. Immunofluorescence reported in literature (PMID: 22269650).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 59% PBS (pH 7.2), 0.05% BSA and 40% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Protein A purified

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for Integrin beta 1/CD29 Antibody (EP1041Y)

• CD29 antigen
• CD29
• Fibronectin receptor subunit beta
• FNRBVLAB
• GPIIA
• Integrin beta 1
• integrin beta-1
• integrin VLA-4 beta subunit
• integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12)
• ITGB1
• MDF2
• MSK12
• very late activation protein, beta polypeptide
• VLA-4 subunit beta
• VLA-BETA

Background

Integrin beta-1 (ITGB1, CD29, VLA-beta) is the beta subunit found in the integrin families, forming a heterodimer integrin receptor through non-covalent bonding with various integrin alpha subunits. Integrin heterodimer containing Integrin beta-1 binds to various cell surface and extracellular proteins (CD49a-f, CD51) to mediate cell to cell and cell to matrix adhesion (1). Integrin beta-1 plays a critical role in the cell adhesion and recognition in embryogenesis, hemostasis, immune response, tissue repair, metastatic diffusion of tumor cells and development (2, 3, 4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin beta 1/CD29 Antibody (NB110-57123)(8)

Publications using NB110-57123

• Shen Congcong, Sun Linlin, Zhu Ningwen et al. Kindlin-1 contributes to EGF-induced re-epithelialization in skin wound healing. International Journal of Molecular Medicine 2017-01-01 [PMID: 28290610] (WB, WB, Mouse)
• Sakamuri SSVP, Takawale A, Basu R, Fedak PWM et al. Differential Impact of Mechanical Unloading on Structural and Non-Structural Components of the Extracellular Matrix in Advanced Human Heart Failure Translational Research 2016-02-23 [PMID: 26963743] (IHC-P, WB, Human)
• Tsunaki Hongu, Yuji Funakoshi, Shigetomo Fukuhara et al. Arf6 regulates tumour angiogenesis and growth through HGF-induced endothelial beta1 integrin recycling. Nature Communications 2015-01-01 [PMID: 26239146] (WB, Human, Mouse)
• Naydenov NG, Feygin A, Wang L et al. N-Ethylmaleimide-sensitive Factor Attachment Protein A (ASNAP) Regulates Matrix Adhesion and Integrin Processing in Human Epithelial Cells. J Biol Chem 2014-01-24 [PMID: 24311785] (ICC/IF, WB, Human)
• Bell AJ, Satchwell TJ, Heesom KJ et al. Protein Distribution during Human Erythroblast Enucleation In Vitro PLoS One 2013-01-01 [PMID: 23565219] (WB, Human)
• Rankin CR, Hilgarth RS, Leoni G et al. Annexin A2 Regulates beta1 Integrin Internalization and Intestinal Epithelial Cell Migration. J Biol Chem 2013-05-24 [PMID: 23558678] (WB, Human)
• Naydenov NG, Brown B, Harris G, Dohn MR, Morales VM, Baranwal S, Reynolds AB, Ivanov AI. A Membrane Fusion Protein alphaSNAP Is a Novel Regulator of Epithelial Apical Junctions. PLoS One;7(4). 2012-01-01 [PMID: 22485163] (ICC/IF, WB, Human)
• Altrock E, Muth CA, Klein G et al. The significance of integrin ligand nanopatterning on lipid raft clustering in hematopoietic stem cells. Biomaterials. 2012-01-21 [PMID: 22269650]

Review for Integrin beta 1/CD29 Antibody (NB110-57123) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 04/03/2014

Summary

• Application: Western Blot
• Sample Tested: SK-CO15 colonic epithelila cell lysates
• Species: Human
• Lot: Gr 115994-10

FAQs for Integrin beta 1/CD29 Antibody (NB110-57123). (Showing 1 - 1 of 1 FAQs).

1. I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.
   • I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.

Bioinformatics

• Gene Symbol: ITGB1
• Entrez:
  • 3688(Human)
  • 16412(Mouse)
  • 24511(Rat)
• Uniprot:
  • P05556()