Integrin alpha E/CD103 Antibody (ITGAE/3904R) [Janelia Fluor® 525]

• Reactivity: Hu
• Applications: IHC
• Clone: ITGAE/3904R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Janelia Fluor 525

Integrin alpha E/CD103 Antibody (ITGAE/3904R) [Janelia Fluor® 525] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant human Integrin alpha E/CD103 protein fragment (aa 775-855) (exact sequence is proprietary) (Uniprot: P38570 )
• Localization: Cell surface and cytoplasm
• Marker: T-Cell Lymphoma & Hairy Cell Leukemia Marker
• Specificity: This monoclonal antibody recognizes a protein of 150kDa, identified as CD103, which is the alpha-E integrin subunit of the heterodimeric alpha-E beta-7 (aEb7) integrin belonging to a small beta-7 integrin subfamily. CD103 is expressed on more than 95% of intraepithelial CD8+ cells and on 40% of mucosa-associated T cells, whereas less than 2% of resting blood lymphocytes are CD103-positive. In several malignant conditions, such as T-cell lymphomas and hairy cell leukemia (HCL), the cells express CD103. Antibody to CD103 is an extremely useful addition to the IHC panel for the diagnosis of hairy cell leukemia (HCL).
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ITGAE
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NBP3-08855. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Integrin alpha E/CD103 Antibody (ITGAE/3904R) [Janelia Fluor® 525]

• antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide
• CD103 antigen
• CD103
• HML-1 antigen
• HUMINAE
• Integrin alpha E
• integrin alpha-E
• Integrin alpha-IEL
• integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alphapolypeptide)
• ITGAE
• MGC141996
• Mucosal lymphocyte 1 antigen

Background

CD103 is a member of the integrin series of adhesion molecules. This antigen defines a developmentally important subset of T cells, namely mucosal T cells including all IEL (intraepithelial lymphocytes) and approx. 20% of lamina propria T cells. Expression of CD103 is more restricted outside these mucosal organs, appearing at lower levels on T cell subsets of the lymph node, dendritic epidermis and periphery. In non-epithelial CD103+ T cells there is a bias toward expression on CD8+.cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITGAE