Integrin alpha E/CD103 Antibody (2E7) [PE]

Flow Cytometry: Integrin alpha E/CD103 Antibody (2E7) [PE] [NBP1-28126] - BALB/c mouse mesenteric lymph node cells were stained with Hamster Anti-Mouse CD103-PE and Rat Anti-Mouse CD3 epilson-FITC.

• Reactivity: Mu
• Applications: Flow
• Clone: 2E7
• Clonality: Monoclonal
• Host: Armenian Hamster
• Conjugate: PE
• Concentration: 0.1 mg/ml

Integrin alpha E/CD103 Antibody (2E7) [PE] Summary

• Immunogen: C57BL/6J mouse intestinal intraepithelial lymphocytes (IEL)
• Specificity: The CD103/alpha IELbeta7 integrin complex (Mr 160 and 115 kDa peptides).
• Isotype: IgG2
• Clonality: Monoclonal
• Host: Armenian Hamster
• Gene: ITGAE
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.2 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS and a stabilizing agent
• Preservative: Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein A or G purified

Notes

Protect conjugated forms from light.

Alternate Names for Integrin alpha E/CD103 Antibody (2E7) [PE]

• antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide
• CD103 antigen
• CD103
• HML-1 antigen
• HUMINAE
• Integrin alpha E
• integrin alpha-E
• Integrin alpha-IEL
• integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alphapolypeptide)
• ITGAE
• MGC141996
• Mucosal lymphocyte 1 antigen

Background

CD103/Integrin alpha E is a member of the integrin series of adhesion molecules. This antigen defines a developmentally important subset of T cells, namely mucosal T cells including all IEL (intraepithelial lymphocytes) and approx. 20% of lamina propria T cells. Expression of CD103 is more restricted outside these mucosal organs, appearing at lower levels on T cell subsets of the lymph node, dendritic epidermis and periphery. (1-3) In non-epithelial CD103+ T cells there is a bias toward expression on CD8+.cells. The 2E7 mAb exhibits antigen immunoprecipitation patterns similar if not identical to the rat anti-mouse CD103 mAb M290. (1) The 2E7 mAb is reported to have signal-inducing activity in a redirected lysis assay and to co-stimulate IEL and CD8+ lymph node cells in conjunction with anti-TCR in vitro, which runs counter to the usual inhibition of CTL by anti-integrins. (1) This co-stimulatory activity is also shared by the mAb M290. (4) The property of costimulation is not unique to the mouse since the combination of anti-human alpha IEL plus anti-TCR will produce similar proliferation in human cells, with the exception that CD8+ cells are not stimulated. (5)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITGAE