Integrin alpha 2/CD49b Antibody (CL7318) - BSA Free

Immunohistochemistry-Paraffin: Integrin alpha 2/CD49b Antibody (CL7318) [NBP2-76483] - Analysis in human skin and skeletal muscle tissues. Corresponding ITGA2 RNA-seq data are presented for the same tissues.

Western Blot: Integrin alpha 2/CD49b Antibody (CL7318) [NBP2-76483] - Analysis in human cell line RT-4.

Immunohistochemistry-Paraffin: Integrin alpha 2/CD49b Antibody (CL7318) [NBP2-76483] - Staining of human prostate shows moderate membranous postivity in glandular cells.

Immunohistochemistry-Paraffin: Integrin alpha 2/CD49b Antibody (CL7318) [NBP2-76483] - Staining of human skin shows strong membranous positivity in cells in basal layer of epidermis.

Immunohistochemistry-Paraffin: Integrin alpha 2/CD49b Antibody (CL7318) [NBP2-76483] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.

Immunohistochemistry-Paraffin: Integrin alpha 2/CD49b Antibody (CL7318) [NBP2-76483] - Staining of human small intestine shows moderate to strong membranous positivity in glandular cells.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL7318
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Integrin alpha 2/CD49b Antibody (CL7318) - BSA Free Summary

• Immunogen: This antibody was developed using a recombinant protein derived from P17301, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGA2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (80%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.2, containing 40% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for Integrin alpha 2/CD49b Antibody (CL7318) - BSA Free

• alpha-2 subunit
• CD49b antigen
• CD49b
• Integrin alpha 2
• integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor)
• ITGA2
• VLA 2
• VLA-2 alpha

Background

Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. They are responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. Aberrant integrin expression has been found in many epithelial tumours. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITGA2