Integrin alpha 2/CD49b Antibody (31H4) - BSA Free

• Reactivity: Hu
• Applications: ELISA, Flow, IP, CyTOF-ready
• Clone: 31H4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Integrin alpha 2/CD49b Antibody (31H4) - BSA Free Summary

• Description: Novus Biologicals Mouse Integrin alpha 2/CD49b Antibody (31H4) - BSA Free (NBP2-50145) is a monoclonal antibody validated for use in ELISA, Flow and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified human b1 integrin preparation from HT1080 fibrosarcoma cell extract.
• Specificity: It is specific to the alpha 2 subunit by relative expression of antigen on various cell lines by Flow Cytomery, and its recognition of affinity purified alpha 2 beta 1 in Dot Blot.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGA2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:10 - 1:1000
  • Immunoprecipitation 1:10 - 1:500
• Application Notes: Capable of immunoprecipitating the Integrin alpha 2/CD49b subunit from human cell-lines surface labelled with 125I. It has been confirmed specific to Integrin alpha 2/CD49b by relative expression of antigen on various cell lines by flow cytometry, and recognition of affinity purified Integrin alpha 2 beta 1 in dot blots.This antibody is Cytof ready.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Integrin alpha 2/CD49b Antibody (31H4) - BSA Free

• alpha-2 subunit
• CD49b antigen
• CD49b
• Integrin alpha 2
• integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor)
• ITGA2
• VLA 2
• VLA-2 alpha

Background

Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. They are responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. Aberrant integrin expression has been found in many epithelial tumours. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITGA2