Integrin alpha 10 Antibody (885501) [Unconjugated]

C2C12 mouse myoblast cell line was stained with Rat Anti-Mouse Integrin alpha 10 Monoclonal Antibody (Catalog # MAB9757, filled histogram) or Rat IgG2A isotype control antibody (Catalog # MAB006, open histogram) followed by Goat anti-Rat IgG APC-conjugated secondary antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow, CyTOF-ready
• Clone: 885501
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

Integrin alpha 10 Antibody (885501) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin alpha 10 beta 1
  Phe23-Thr1119 (Integrin alpha 10) and Gln21-Asp728 (Integrin beta 1)
  Accession # NP_001289400.1 (Integrin alpha 10) and P09055 (Integrin beta 1)
• Specificity: Detects mouse Integrin alpha 10 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Intagrin alpha 2 beta 1 was observered.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin alpha 10 Antibody (885501) [Unconjugated]

• Integrin alpha 10
• integrin alpha-10
• integrin, alpha 10
• ITGA10
• PRO827

Background

Integrin alpha 10 beta 1 is one of twelve integrin family adhesion receptors that share the beta 1 subunit (1‑3). The non‑covalent heterodimer of 160 kDa alpha 11 and 130 kDa beta 1/CD29 type I transmembrane glycoprotein subunits is expressed mainly on chondrocytes within cartilage, but also in fibrous connective tissues such as heart valves and ligaments (3, 4). The alpha 10 extracellular domain (ECD) contains an I (inserted) domain which includes the ligand binding site (2, 3, 5). The beta 1 ECD contains a vWFA domain, which participates in binding. Each subunit then has a transmembrane sequence and a short cytoplasmic tail. The dimer is folded when it is least active. Divalent cations and intracellular (inside‑out) signaling convert it to its most active, extended and open conformation (1, 2). The 1100 amino acid (aa) mouse  alpha 10 extracellular domain (ECD) shares 96% aa sequence identity with  rat and 88‑89% with human, rabbit, porcine, canine and bovine alpha 10, while the 708 aa mouse beta 1 ECD shares 98% aa identity with rat and 93‑94% with human, bovine, porcine, ovine, canine and feline beta 1. A potential mouse alpha 10 splice variant diverges at aa 1039 and is terminated prematurely. If translated, this variant would result in a secreted protein (6). I domain‑containing beta 1 integrins alpha 1 beta 1, alpha 2 beta 1, alpha 10 beta 1 and alpha 11 beta 1 all bind collagens; all but alpha 11 beta 1 also bind laminins (5, 7, 8). During cartilage differentiation, alpha 10 beta 1 is thought to be the main integrin binding type II and IX cartilage collagens (3‑5, 7‑10). However, deletion of mouse alpha 10 causes a mild phenotype including slightly shortened bones and narrowed hypertrophic zones, indicating that another collagen‑binding integrin, likely alpha 2 beta 1, may compensate for alpha 10 beta 1 functions (11). Migration of melanoma cells has been noted to correlate with alpha 10 beta 1 expression (12).

1. Takada, Y. et al. (2007) Genome Biol. 8:215.
2. Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
3. Camper, L. et al. (1998) J. Biol. Chem. 273:20383.
4. Camper, L. et al. (2001) Cell Tiss. Res. 306:107.
5. Tulla, M. et al. (2001) J. Biol. Chem. 276:48206.
6. Bengtsson, T. et al. (2001) Matrix Biol. 20:565.
7. McCall-Culbreath, K.D. and M.M. Zutter (2008) Curr. Drug Targets 9:139.
8. Popova, S.N. et al. (2007) Acta Physiol. 190:179.
9. Varas, L. et al. (2007) Stem Cells Dev. 16:965.
10. Gigout, A. et al. (2008) J. Biol. Chem. 283:31522.
11. Bengtsson, T. et al. (2004) J. Cell Sci. 118:939.
12. Wenke, A.K. et al. (2007) Cell Oncol. 29:373.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • NP_001289400.1 (Integrin alpha 10) and P09055 (Integrin()