Insulin R/CD220 Antibody (MA-20) [FITC]

Product Discontinued

Insulin R/CD220 Antibody (MA-20) [FITC] Summary

• Immunogen: Highly purified Insulin Receptor (alpha-subunit) from human term placentas. [UniProt# P06213]
• Localization: Membrane; single pass type I membrane protein.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: INSR
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • ELISA
  • Immunoprecipitation
  • Western Blot
• Application Notes: This Insulin Receptor (MA-20) antibody is useful for ELISA, Immunoprecipitation, Western blot and Blocking/Neutralizing assays.

Reactivity Notes

Human and rat. Mouse reactivity reported in scientific literature (PMID: 24389353)

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for Insulin R/CD220 Antibody (MA-20) [FITC]

• CD 220
• CD220 antigen
• CD220
• EC 2.7.10
• EC 2.7.10.1
• HHF5
• INSR
• Insulin R
• insulin receptor
• InsulinR
• IR

Background

Insulin receptor (INSR) is a receptor tyrosine kinase which is well known for its regulatory role in peripheral glucose metabolism. INSR contain disulfide bridged 135-kD alpha subunits (extracellular ligand binding portion) and 95-kD beta subunit (an extracellular, a single transmembrane, and an intracellular kinase domain). During signaling, ligand binding to alpha subunits activates the intrinsic kinase activity located in the beta subunits and subsequently initiates a cascade of phosphorylation events leading to various biological functions. Unlike other RTKs, INSR's most activities require accessory molecules called insulin receptor substrates (IRSs) e.g. example IRS1-4 is required to engage multiple downstream signaling. INSR kinase can activate two major cellular signaling pathways, PI3K/Akt and Ras/MAPK, which regulate diverse cellular processes including gene expression, protein synthesis, and vesicle trafficking, which result in the regulation of glucose, lipid and protein metabolism, cell growth and differentiation. Defects in INSR have been linked to several pathologies including Rabson-Mendenhall syndrome (RMS), leprechaunism (LEPRCH), noninsulin-dependent diabetes mellitus (NIDDM), familial hyperinsulinemic hypoglycemia type 5 (HHF5) and insulin-resistant diabetes mellitus with acanthosis nigricans type A (IRAN type A).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: INSR