Insulin R/CD220 [p Tyr1185] Antibody (EP351(2)Y)

Product Discontinued

Insulin R/CD220 [p Tyr1185] Antibody (EP351(2)Y) Summary

• Immunogen: A phospho synthetic peptide corresponding to residues surrounding Tyrosine 1185 of human INSR was used as an immunogen.
• Modification: p Tyr1185
• Specificity: This antibody detects INSR phosphorylated at Tyrosine 1185.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: INSR
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-250
  • Immunoprecipitation 1:20
  • Western Blot 1:500
• Application Notes: This product is useful for: Western Blot, Immunocytochemistry, Immunoprecipitation. In Western blot this antibody detects a band at approximately 95kDa.

Reactivity Notes

Reacts with: Human Does not react with Mouse, Rat

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 49% PBS, 0.05% BSA and 50% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Tissue culture supernatant

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for Insulin R/CD220 [p Tyr1185] Antibody (EP351(2)Y)

• CD 220
• CD220 antigen
• CD220
• EC 2.7.10
• EC 2.7.10.1
• HHF5
• INSR
• Insulin R
• insulin receptor
• InsulinR
• IR

Background

The insulin receptor is an essential protein present on the surface of virtually all cells. Little is known about the control of the level of this protein on cellular surfaces, but it has been found that the level of insulin receptor protein correlates roughly with the level of insulin receptor (IR) gene transcripts within cells (1). The insulin receptor plays a critical role in the maintenance of glucose homeostasis. Regulation of this key function must be under stringent controls (2). Insulin receptor substrate 1 (IRS-1) and src homology and collagen protein (SHC) are signaling proteins which are rapidly phosphorylated on tyrosines after insulin receptor activation. It has been shown that both SHC and IRS-1 interact with the tyrosine-phosphorylated NPEY motif of the IR and insulin-like growth factor I receptor via non-SH2 domains (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: INSR
• Entrez:
  • 3643(Human)
• Uniprot:
  • P06213()