Inositol Monophosphatase 3/IMPAD1 Antibody

Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Inositol Monophosphatase 3/IMPAD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7028) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Inositol Monophosphatase 3/IMPAD1 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Inositol Monophosphatase 3/IMPAD1 was detected in immersion fixed mouse mesenchymal stem cells differentiated into chondrocytes using Sheep Anti-Mouse/Rat Inositol Monophosphatase 3/IMPAD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7028) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Inositol Monophosphatase 3/IMPAD1 Antibody Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse Inositol Monophosphatase 3/IMPAD1
  Asn145-His356
  Accession # Q80V26
• Specificity: Detects recombinant mouse Inositol Monophosphatase 3/IMPAD1 in direct ELISAs and Western blots. Detects mouse Inositol Monophosphatase 3/IMPAD1 and rat Inositol Monophosphatase 3/IMPAD1 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: IMPAD1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Inositol Monophosphatase 3/IMPAD1 Antibody

• EC 3.1.3
• EC 3.1.3.25
• FLJ20421
• IMP 3
• IMP3
• IMPA3
• IMPA3IMPase 3
• IMPAD1
• IMPase 3
• inositol monophosphatase 3
• inositol monophosphatase domain containing 1
• Inositol monophosphatase domain-containing protein 1
• Inositol-1(or 4)-monophosphatase 3
• Myo-inositol monophosphatase A3

Background

IMPAD1 (Inositol monophosphatase domain-containing protein 1; also IMPA3, gPAPP and IMPase 3) is a 40-42 kDa member of the inositol monophosphatase family of proteins. It is expressed in embryo, and found in Purkinje cells, brain stem, lung and chondrocytes. IMPAD1 in theory may catalyze the synthesis of myo-inositol from myo-inositol monophosphate. Free myo-inositol is used to generate inositol phospholipid, an essential component of intracellular signaling pathways that mobilize calcium. IMPAD1 is reported to promote sulfation of chondroitin by converting PAP (an endproduct of the sulfation process) to 5'-AMP. PAP is a known inhibitor of SULTs. IMPAD1 is a 356 amino acid (aa) type II transmembrane Golgi-embedded glycoprotein. It contains a short cytoplasmic tail (aa 1-12) and an extended luminal region (aa 34-356) that contains its catalytic domain (aa 60-347). Over aa 145-356, mouse IMPAD1 shares 99% and 93% aa sequence identity with rat and human IMPAD1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Inositol Monophosphatase 3/IMPAD1 Antibody (AF7028)(1)

Publication using AF7028

• R Bajaj, BL Rodriguez, WK Russell, AN Warner, L Diao, J Wang, MG Raso, W Lu, K Khan, LS Solis, H Batra, X Tang, JF Fradette, ST Kundu, DL Gibbons Impad1 and Syt11 work in an epistatic pathway that regulates EMT-mediated vesicular trafficking to drive lung cancer invasion and metastasis Cell Reports, 2022-09-27;40(13):111429. 2022-09-27 [PMID: 36170810] (ICC, IHC, Western Blot, Mouse)

Bioinformatics

• Gene Symbol: IMPAD1
• Uniprot:
  • Q80V26()